The changes of telomerase activity and proliferating cell nuclear antigen(PCNA) expression in the developmental stages of rat's upper digestive system.
- Author:
Iel Yong SUNG
1
;
Jong Ryoul KIM
Author Information
1. Dept. of Oral & Maxillofacial Surgery, College of Medicine, Ulsan University. ielyongsung@hanmail.net
- Publication Type:Original Article
- Keywords:
Telomerase;
PCNA;
Upper digestive system
- MeSH:
Aging;
California;
Clone Cells;
Digestive System*;
Enzyme-Linked Immunosorbent Assay;
Fetus;
Gingiva;
Immunohistochemistry;
Mouth Mucosa;
Mucous Membrane;
Nitrogen;
Palate;
Parotid Gland;
Parturition;
Polymerase Chain Reaction;
Proliferating Cell Nuclear Antigen;
Rats, Sprague-Dawley;
Salivary Glands;
Telomerase*;
Tongue
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2004;30(2):85-99
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: This study observed the changes in the telomerase activity, it's developmental regulation, PCNA expression, and their correlation in rat's upper digestive organs during growth and aging. MATERIALS AND METHODS: Upper digestive organs(buccal mucosa, gingiva, palate, submandibular and parotid glands, and tongue) were aseptically removed from Sprague-Dawley rats of fetal(gestational 20 days), growing(1, 2, 3, 5, and 7 weeks after birth) and adult(12 week old). Samples for telomerase activity were frozen on liquid nitrogen immediately after sacrifice, and stored until the use at -75 degree C in order to measure it. Telomerase activity was measured by a PCR-based telomeric repeat amplication protoco(TRAP) assay and quantitated with Photometric Telo TAGGG Telomerase PCR ELISA plus(Roche Diagnostics GmbH. Mannheim. ermany). PCNA expression were measured immunohistochemistry with anti PCNA Ab-1, Clone PC10(NeoMark. California. USA). RESULTS: 1. Telomerase activities in buccal mucosa, palate and gingiva were the highest in fetus and decreased gradually or rapidly after birth and then diminished, but In salivary gland and tongue were the highest in fetus and also high at 1 week and then decreased rapidly. 2. PCNA expression in buccal mucosa, gingiva, Tongue and salivary gland was the highest in fetus and decreased gradually and then diminished. but only in palate decreased rapidly after birth and then diminished. CONCLUSION: The highest telomerase activity of embryonic stage decreased rapidly after birth in rat's upper digestive organs. There may be a developmental regulation of telomerase activity, but not a tissue-specific. This telomerase activity seems correlated closely with PCNA expression in rat's upper digestive system.
