The most appropriate antimitotic treatment of Ara-C in schwann cell-enriched culture from dorsal root ganglia of new born rat.
- Author:
Soung Min KIM
1
;
Jong Ho LEE
;
Kang Min AHN
;
Nam Yeol KIM
;
Mi Ae SUNG
;
Soon Jeong HWANG
;
Ji Hyuck KIM
;
Jeong Won JAHNG
Author Information
1. Department of Oral & Maxillofacial Surgery, College of Dentistry, Seoul National University. leejongh@plaza.snu.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Schwann cells;
dorsal root ganglia;
S-100;
GFAP;
Ara-C
- MeSH:
Adult;
Animals;
Cell Count;
Cell Culture Techniques;
Cytarabine*;
Fibroblasts;
Ganglia, Spinal*;
Humans;
Neurons;
Peripheral Nervous System;
Rats*;
Schwann Cells;
Spinal Nerve Roots*
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2004;30(2):100-107
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Schwann cell, one of important components of peripheral nervous system, interact with neurons to mutually support the growth and replication of embryonal nerves and to maintain the different functions of adult nerves. The Ara-C, known as an antimitotic agent, have been used to have high effectiveness in eliminating fibroblasts during Schwann cell culture period. This enrichment effect is also known to be cummulative with each successive pulse of Ara-C applied and is due to a progressive loss of fibroblasts. But the cytotoxicity by Ara-C is also cummulative and noticeable over the period. To determine the most effective application time and interval of Ara-C in the Schwann cell culture, we observed the Schwann cell purity and density with the Ara-C treatment in plain and three-dimensional culture from dorsal root ganglion of new born rat. By culturing dispersed dorsal root ganglia, we can repeatedly generate homogenous Schwann cells, and cellular morphology and cell count with mean percentages were evaluated in the plain culture dishes and in the immunostainings of S-100 and GFAP in the three-dimensional culture. The Ara-C treated cultures showed a higher Schwann cell percentage (31.0%+/-8.09% in P4 group to 65.5%+/-24.08% in P2 group), compared with that obtained in the abscence of Ara-C (17.6%+/-6.03%) in the plain culture after 2 weeks. And in the three-dimensional culture, S-100 positive cells increased to 56.22%+/-0.67% and GFAP positive cells to 66.46%+/-1.83% in G2 group (p<0.05), higher yield than other groups with Ara-C application. Therefore, we concluded that the Ara-C treatment is effective for the proliferation of Schwann cells contrast to the fibroblasts in vitro culture, and the first application after 24 hours from cell harvesting and subsequent 2 pulse treatment (P2 group in plain culture and G2 group in three-dimensional culture) was more effective than other application protocols.
