Curcumin attenuates the middle cerebral artery occlusion-induced reduction in gamma-enolase expression in an animal model.
10.5625/lar.2015.31.4.198
- Author:
Sang Ah GIM
1
;
So Ra LEE
;
Fawad Ali SHAH
;
Phil Ok KOH
Author Information
1. Department of Anatomy, College of Veterinary Medicine, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Korea. pokoh@gnu.ac.kr
- Publication Type:Brief Communication
- Keywords:
gamma-enolase;
curcumin;
neuroprotection
- MeSH:
Adult;
Animals*;
Blotting, Western;
Brain Ischemia;
Cerebral Cortex;
Curcumin*;
Humans;
Infarction, Middle Cerebral Artery;
Male;
Middle Cerebral Artery*;
Models, Animal*;
Neurons;
Neuroprotective Agents;
Phosphopyruvate Hydratase*;
Polymerase Chain Reaction;
Proteomics;
Rats
- From:Laboratory Animal Research
2015;31(4):198-203
- CountryRepublic of Korea
- Language:English
-
Abstract:
Curcumin exerts a protective effect in cerebral ischemia through its anti-oxidant and anti-inflammatory activities. gamma-enolase is a glycolytic enzyme expressed in neurons that is known to exerts a neuroprotective effect. We investigated whether curcumin regulates gamma-enolase expression in focal cerebral ischemic injury in rats. Middle cerebral artery occlusion (MCAO) was performed to induce focal cerebral ischemia. Adult male rats were injected intraperitoneally with either vehicle or curcumin (50 mg/kg) 1 h after MCAO and cerebral cortex tissues were isolated 24 h after MCAO. We found that MCAO-induced injury resulted in a reduction in gamma-enolase expression in vehicle-treated animals using a proteomics approach. However, this reduction was attenuated in animals with MCAO treated with curcumin. Reverse-transcription PCR and Western blot analyses also showed that curcumin treatment prevented the MCAO injury-induced reduction in gamma-enolase expression. The results of this study suggest that curcumin exerts its neuroprotective function in focal cerebral ischemia by regulating the expression of gamma-enolase.
