Establishment of hydrochloric acid/lipopolysaccharide-induced pelvic inflammatory disease model.
10.4142/jvs.2016.17.3.413
- Author:
Yeonsu OH
1
;
Jaehun LEE
;
Hyeon Cheol KIM
;
Tae Wook HAHN
;
Byung Il YOON
;
Jeong Hee HAN
;
Yong Soo KWON
;
Joung Jun PARK
;
Deog Bon KOO
;
Ki Jong RHEE
;
Bae Dong JUNG
Author Information
1. College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Korea. bdjung@kangwon.ac.kr
- Publication Type:Original Article
- Keywords:
hydrochloric acid;
lipopolysaccharide;
mice;
pelvic inflammatory disease;
pelvic inflammatory disease model
- MeSH:
Animals;
Bacteria;
Blotting, Western;
Coinfection;
Cytokines;
Endometrium;
Enzyme-Linked Immunosorbent Assay;
Female;
Gram-Negative Bacteria;
Humans;
Hydrochloric Acid;
Inflammation;
Interleukin-6;
Interleukins;
Lung;
Mice;
Models, Animal;
Necrosis;
Neutrophils;
Pelvic Inflammatory Disease*;
Peritoneum;
Pregnancy;
Sepsis;
Sexually Transmitted Diseases;
Stomach;
Uterus
- From:Journal of Veterinary Science
2016;17(3):413-419
- CountryRepublic of Korea
- Language:English
-
Abstract:
Pelvic inflammatory disease (PID), which is one of the most problematic complications experienced by women with sexually transmitted diseases, frequently causes secondary infections after reproductive abnormalities in veterinary animals. Although the uterus is self-protective, it becomes fragile during periods or pregnancy. To investigate PID, bacteria or lipopolysaccharide (LPS) extracted from gram negative bacteria has been used to induce the disease in several animal models. However, when LPS is applied to the peritoneum, it often causes systemic sepsis leading to death and the PID was not consistently demonstrated. Hydrochloric acid (HCl) has been used to induce inflammation in the lungs and stomach but not tested for reproductive organs. In this study, we developed a PID model in mice by HCl and LPS sequential intracervical (i.c.) administration. The proinflammatory cytokines, interleukin (IL)-1β, IL-6 and tumor necrosis factor-α, were detected in the mouse uterus by western blot analysis and cytokine enzyme-linked immunosorbent assay after HCl (25 mg/kg) administration i.c. followed by four LPS (50 mg/kg) treatments. Moreover, mice exhibited increased infiltration of neutrophils in the endometrium and epithelial layer. These results suggest that ic co-administration of HCl and LPS induces PID in mice. This new model may provide a consistent and reproducible PID model for future research.
