Expression of embryonic stem cell markers in human term placenta.
- Author:
Gui Se Ra LEE
1
;
Dong Chul KIM
;
Sae Kyung CHOI
;
Jong Chul SHIN
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Catholic University of Korea, Seoul, Korea. cshin@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Placenta;
Embryonic stem cell markers
- MeSH:
Amnion;
Antibodies;
Antigens, Surface;
Antigens, Tumor-Associated, Carbohydrate;
Chorion;
Embryonic Stem Cells;
Epithelial Cells;
Humans;
Mesenchymal Stromal Cells;
Placenta;
Pluripotent Stem Cells;
Pregnancy;
Rejection (Psychology);
Stage-Specific Embryonic Antigens;
Stem Cells
- From:Korean Journal of Obstetrics and Gynecology
2009;52(7):700-706
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To determine which compartments of placenta in the term pregnancy express the embryonic stem cell markers. METHODS: We have used immunohistochemical methods with antibodies to embryonic stem cell surface antigens, TRA 1-60 (Tumor rejection antigen 1-60), TRA 1-81 (Tumor rejection antigen 1-81), SSEA-3 (stage-specific embryonic antigen-3) and SSEA-4 (stage-specific embryonic antigen-4), to identify and localize stem cells in the term placenta. RESULTS: Stem cell marker-positive cells were found in all layer of placenta. Amnionic epithelial cells was immunoreactive with TRA 1-60, TRA 1-81. Amnionic mesenchymal stromal cells was immunoreactive with TRA 1-81. Chorionic mesenchymal stromal cells was immunoreactive with TRA 1-60 and TRA 1-81. SSEA-3 and SSEA-4 were not stained at any compartment of the term placenta. Compartment that was stained most strongly by TRA 1-60 was the amnionic epithelial cells layer. Compartment that was stained most strongly by TRA 1-81 was the chorionic mesenchymal stromal cells layer. CONCLUSION: The mesenchymal stroma cells of the amnion and chorion as well as amnionic epithelial cells may be useful source of pluripotent stem cells in the term placenta.
