Nestin Expression in the Adult Mouse Retina with Pharmaceutically Induced Retinal Degeneration.
10.3346/jkms.2017.32.2.343
- Author:
Chan Hee MOON
1
;
Heeyoon CHO
;
Yoon Kyung KIM
;
Tae Kwann PARK
Author Information
1. Department of Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Nestin;
N-methyl-N-nitrosourea (MNU);
Retinal Degeneration;
Mouse
- MeSH:
Adult*;
Animals;
Blotting, Western;
Cell Count;
Glial Fibrillary Acidic Protein;
Gliosis;
Humans;
Injections, Intraperitoneal;
Methylnitrosourea;
Mice*;
Microglia;
Nestin*;
Retina*;
Retinal Degeneration*;
Retinaldehyde*;
Up-Regulation
- From:Journal of Korean Medical Science
2017;32(2):343-351
- CountryRepublic of Korea
- Language:English
-
Abstract:
The present study investigated the temporal pattern and cellular localization of nestin in the adult mouse retina with pharmaceutically induced retinal degeneration using N-methyl-N-nitrosourea (MNU). After a single intraperitoneal injection of MNU in 8-week-old C57BL/6 mice, the animals were sacrificed at 1, 3, 5, 7, and 21 days (n = 6, in each stage). The eyes were examined by means of immunohistochemical tests using nestin, ionized calcium-binding adaptor molecule (Iba-1), CD11b, F4/80, and glial fibrillary acidic protein (GFAP). Western blot analysis and manual cell counting were performed for quantification. Nestin expression was increased after MNU administration. Nestin+/Iba-1+ cells were migrated into outer nuclear layer (ONL) and peaked at day 3 post injection (PI). Nestin+/CD11b+ cells were also mainly identified in ONL at day 3 PI and peaked at day 5. Nestin+/F4/80+ cells were shown in the subretinal space and peaked at day 3 PI. Nestin+/GFAP+ cells were distinctly increased at day 1 PI and peaked at day 5 PI. The up-regulation of nestin expression after MNU administration in adult mouse retinal microglia, and monocyte/macrophage suggests that when retinal degeneration progresses, these cells may revert to a more developmentally immature state. Müller cells also showed reactive gliosis and differentiational changes.
