The difference of interieukin - 4 and interferon - r production of Der p I stimulated T cells and effects of immunomodulator in house dust mite sensitive atopic and non - atopic individuals.
- Author:
Jae Won OH
;
Ha Baik LEE
;
Yong Hoon CHUNG
;
Yong CHOI
;
Mi Kyoung SONG
- Publication Type:In Vitro ; Original Article
- Keywords:
interleukin - 4;
interferon - y;
budesonide;
cyclosporin A;
disodium cromoglycate;
atopy
- MeSH:
Allergens;
Bias (Epidemiology);
Budesonide;
Cromolyn Sodium;
Cyclosporine;
Dust*;
Flow Cytometry;
Humans;
Immunologic Factors;
Interferons*;
Interleukin-4;
Lymphocytes;
Pyroglyphidae*;
T-Lymphocytes*;
Tissue Donors
- From:Journal of Asthma, Allergy and Clinical Immunology
1999;19(4):548-556
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Allergic diseases are characterized by immediate - and late - phase reactions to various allergens by the selective activation of a subset of CD4 + T cells. In response to allergen, T cells isolated from atopic donors are biased to low levels of IFN - y and high levels of IL - 4, and vice versa by T cells from non - atopic donor. Objective : The aim of this study was to evaluate the patterns of IL - 4 and IFN - y production after Der p I stimulation and the effect on the cytokine production from T cells by budesonide, disodium cromoglycate and cyclosporin A in atopic and non - atopic individuals. MATERIAL AND METHOD: Seven Der p I specific atopic and 7 non - atopic individuals were selected. We decided the 50% inhibiting concentration of each immunomodulator by lymphocyte proliferation assay, and measured their effects on the cytokine production in vitro by intracellular IL - 4, IFN - y staining and flow cytometry. Results and CONCLUSION: There was significant difference on stimulation index ( SI ) of production of IFN - y as well as IL-4 after Der p I stimulation between atopic and non-atopic individuals ( IL - 4 ; 1.57 +/- 0.7 : 0.98 +/- 0.2, p = 0.026, IFN - r : 1.45 +/- 0.5 : 0.95 +/- 0.2, p = 0.048 ). The synthesis of IL - 4, and IFN - r were significantly inhibited after the stimulation of every immunomodulators in atopic individuals, DSCG couldnt inhibit IL - 4 and IFN - r in nonatopic individuals. There was no significant difference in the inhibiting effect of these immunomodulators in both of them.
