Expression of Vascular Endothelial Growth Factor Receptor mRNA in Eutopic and Ectopic Endometrial Tissues of Patients with Endometriosis.
- Author:
Jeong Yeol PARK
1
;
Chung Hoon KIM
;
Seok Ho HONG
;
Young Jin LEE
;
Seong Wha HONG
;
Hye Eun KWON
;
Sung Hoon KIM
;
Hee Dong CHAE
;
Byung Moon KANG
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan, Asan Medical Center, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Endometriosis;
Angiogenesis;
VEGF;
VEGFR
- MeSH:
Biopsy;
Choristoma;
Endometriosis*;
Female;
Gene Expression;
Humans;
Infertility;
Laparoscopy;
Laparotomy;
Receptors, Vascular Endothelial Growth Factor*;
RNA, Messenger;
Vascular Endothelial Growth Factor A*;
Vascular Endothelial Growth Factor Receptor-1;
Vascular Endothelial Growth Factor Receptor-3
- From:Korean Journal of Obstetrics and Gynecology
2004;47(3):515-522
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To Investigate the expression of vascular endothelial growth factor receptor-1, -2, and -3 (VEGFR-1, -2, and -3) mRNA in the eutopic and ectopic tissues in women with endometriosis. METHODS: At the time of laparoscopy or laparotomy for endometriosis, infertility or other benign gynecologic conditions, a biopsy specimen was taken from the endometrial cavity and a peritoneal endometriotic implant simultaneously whenever appropriate. For control samples, endometrial tissues were obtained from women without visual evidence of endomtriosis. We employed real time quantitative RT-PCR to quantify VEGFR gene expression of these tissues. Comparisions between each group were made using ANOVA (analysis of variance) and Kruskal-Wallis test and statistical significance was defined as p<0.05. RESULTS: The expression of VEGFR-1, -2, and, -3 mRNA was significantly higher in both eutopic and ectopic endometrial tissues of women with endometriosis than in control endometrial tissues during both proliferative and mid-secretory phase. In ectopic endometrial tissue, VEGFR-1 mRNA expression was significantly increased during the mid-secretory phase compared to the proliferative phase. There was a marked increase especially in VEGFR-3 mRNA expression in ectopic endometriotic lesions during the proliferative phase but its expression decreased during the mid-secretory phase. CONCLUSION: mRNA for VEGFR-1, -2, and -3 in an endometriotic lesions might be differentially expressed and their expression appears to be associated with the development of endometriosis.
