Ultastructural study of the fibroblast remodeling in the radiation: impaired wound healing in the tongue of the rat.
- Author:
Jin Koo LEE
1
;
Soon Chul CHOI
;
Tae Won PARK
;
Dong Soo YOU
Author Information
1. Department of Oral and Maxillofacial Radiology, College of Dentistry, Seoul National University, Korea.
- Publication Type:Original Article
- MeSH:
Animal Experimentation;
Animals;
Collagen;
Cytoplasm;
Fibroblasts*;
Humans;
Logic;
Mitochondria;
Models, Theoretical;
Rats*;
Tongue*;
Vacuoles;
Wound Healing*;
Wounds and Injuries*
- From:Journal of Korean Academy of Oral and Maxillofacial Radiology
1997;27(1):141-166
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Radiation-imparied wound healing in animal experiments was believed to be an another logical experimental model to understand the wound healing mechanism in patients. the purpose of this study was to reveal the block point which would result impaired healing. Twenty four rats(Sprague-Dawley stains) were divided into two gruops according to the time interval between irradiati on and wounding. Group I, observing the healing effect on the 1st day and group II are the healing effects on the 7th d ays after irradiation to the wound of the rat tongue. Experimental animals were sacrificed 3, 6, 12 and 24 hours after w ounding. The specimens were examined by the light microscope and trasmission electron microscope. The following results were obtained: 1. Fibroblasts in both goups showed degenerative changes which were dilated mitochondria and rER, reduced microorganell e, vacuoles and little cytoplasmic process. 2. Average length between bands and quantity of the newly produced collagen fibers aroud fibroblasts remained unchanged against control group. 3. The severity of degenerative change of the fibroblast and impairment of wond healing including shortening of the thi ckness of collagen fibers were more sever in the group II than in the group I.
