Immunogenicity and protective efficacy of solubilized merozoite-enriched Theileria sergenti immmunogens III. Characterization of immunodominant peptides.
10.3347/kjp.1994.32.2.111
- Author:
Byeong Kirl BAEK
;
Byeong Soo KIM
;
Byung Moo RHIM
;
Ho Ill LEE
;
Yong Ho PARK
;
Ibulaimu KAKOMA
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- MeSH:
parasitology-protozoa;
Theileria sergenti;
amino acid sequence;
synthetic peptide;
predicted antigenic value;
amino acid
- From:The Korean Journal of Parasitology
1994;32(2):111-116
- CountryRepublic of Korea
- Language:English
-
Abstract:
Immunoblot analysis utilizing bovine sera from naturally or experimentally infected with Theileria sergenti were used to determine the immunodominant polypeptides of T. sergenti (Korea isolate).The previously recognized major bands, 18 kDa, 29 kDa, 34 kDa, and 45 kDa, were excised after electrophoresis and trasferred to PVDF membrane. The individual bands were sequenced. The 34 kDa polypeptide which was the most antigenic and immunogenic peptide was observed in the Western blot. However, Chou-Fasman prediction sites (antiginic site) for antigen determinants of the 45 kDa,34 kDa, 29 kDa and 18 kDa polypeptide were 6, 4, 2 and 0, respectively. However, the 45 kDa polypeptide showed no reaction with anti-T. sergenti hyperimmune serum.
