The Drug Resistance Profile of Mycobacterium abscessus Group Strains from Korea.
- Author:
Seung Heon LEE
1
;
Hee Kyung YOO
;
Seol Hee KIM
;
Won Jung KOH
;
Chang Ki KIM
;
Young Kil PARK
;
Hee Jin KIM
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: Mycobacterium abscessus group; Drug resistance; Mutation
- MeSH: Anti-Bacterial Agents/pharmacology; Bacterial Proteins/genetics; Clarithromycin/pharmacology; DNA Gyrase/genetics; *Drug Resistance, Bacterial; Humans; Methyltransferases/genetics; Microbial Sensitivity Tests; Mutation; Mycobacterium/drug effects/*isolation & purification; Mycobacterium Infections, Nontuberculous/diagnosis/*microbiology; Republic of Korea; Sequence Analysis, DNA
- From:Annals of Laboratory Medicine 2014;34(1):31-37
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Bacteria of the Mycobacterium abscessus group are the second most common pathogens responsible for lung disease caused by nontuberculous mycobacteria in Korea. There is still a lack of studies investigating the genetic mechanisms involved in M. abscessus resistance to antibiotics other than clarithromycin. This study investigated the characteristics of drug resistance exhibited by M. abscessus clinical isolates from Korea. METHODS: We performed drug susceptibility testing for a total of 404 M. abscessus clinical strains. Subspecies were differentiated by molecular biological methods and examined for mutations in drug resistance-related genes. RESULTS: Of the 404 strains examined, 202 (50.00%), 199 (49.26%), and 3 (0.74%) strains were identified as M. abscessus, M. massiliense, and M. bolletii, respectively. Of the 152 clarithromycin-resistant strains, 6 possessed rrl mutations, while 4 of the 30 amikacin-resistant strains contained rrs mutations, and 5 of the 114 quinolone-resistant strains had gyr mutations. All mutant strains had high minimal inhibitory concentration values for the antibiotics. CONCLUSIONS: Our results showed the distribution of the strains with mutations in drug resistance-related genes was low in the M. abscessus group. Furthermore, we performed drug susceptibility testing and sequence analyses to determine the characteristics of these genes in the M. abscessus group.
