Evaluation of a Novel Array-Based Toxoplasma, Rubella, Cytomegalovirus, and Herpes Simplex Virus IgG Enzyme Linked Immunosorbent Assay and Its Comparison with Virion/Serion Enzyme Linked Immunosorbent Assays.
- Author:
Dongsheng WU
1
;
Yuanjian WU
;
Liuhong WANG
;
Weidong XU
;
Qiao ZHONG
Author Information
- Publication Type:Original Article ; Evaluation Studies ; Research Support, Non-U.S. Gov't
- Keywords: Protein array; ELISA; TORCH test; Agreement
- MeSH: Antibodies, Viral/blood; Cytomegalovirus/immunology/*metabolism; *Enzyme-Linked Immunosorbent Assay; Humans; Immunoglobulin G/*analysis/blood; Protozoan Infections/diagnosis; Reagent Kits, Diagnostic; Rubella virus/immunology/*metabolism; Sensitivity and Specificity; Simplexvirus/immunology/*metabolism; Toxoplasma/immunology/*metabolism; Virion/*immunology/metabolism; Virus Diseases/diagnosis
- From:Annals of Laboratory Medicine 2014;34(1):38-42
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: The dramatic increase in use of the IgG test for toxoplasma, rubella, cytomegalovirus (CMV), and herpes simplex virus (HSV) [TORCH] has led to the requirement for a high-efficiency method that can be used in the clinical laboratory. This study aimed to compare the results of BGI-Array ELISA TORCH IgG (BGI-GBI, China) screening method to those of Virion/Serion TORCH IgG ELISA (Virion/Serion, Germany). METHODS: Serum specimens (n=400) submitted for routine IgG testing by Virion/Serion ELISA were also tested using the BGI-Array ELISA method. The agreements of these two kinds of method were analyzed by kappa-coefficients calculation. RESULTS: Following repeat testing, the BGI-Array ELISA TORCH IgG assays demonstrated agreements of 99.5% (398/400 specimens), 98% (392/400 specimens), 99% (396/400 specimens), and 99.5% (398/400 specimens), respectively. The BGI-Array ELISA IgG assays provided results comparable to Virion/Serion ELISA results, with kappa-coefficients showing near-perfect agreement for the HSV (kappa=0.87), rubella (kappa=0.92) and CMV (kappa=0.93) and substantial agreement for the toxoplasma (kappa=0.80) IgG assays. The use of the BGI-Array ELISA TORCH IgG assays could reduce the turnaround time (1.5 hr vs. 5 hr by Virion/Serion ELISA for 100 specimens) and were easy to use. CONCLUSIONS: BGI-Array ELISA TORCH IgG shows a good agreement with Virion/Serion ELISA methods and is suitable for clinical application.
