Production and characterization of monoclonal antibodies to oxidized LDL.
- Author:
Kyung Ho CHOI
1
;
Hyun Soon LEE
;
Hong Keun CHUNG
Author Information
1. Department of Biochemistry, Seoul National University College of Medicine, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
oxidized LDL;
monoclonal antibodies;
atherosclerosis;
renal glomerular injury
- MeSH:
Antibodies, Monoclonal;
Antibody Affinity;
Antibody Specificity;
Enzyme-Linked Immunosorbent Assay;
Epitopes;
Human;
Lipoproteins, LDL/immunology*;
Malondialdehyde/immunology;
Malondialdehyde/analysis;
Peptide Fragments/immunology;
Thiobarbituric Acid Reactive Substances/analysis
- From:Experimental & Molecular Medicine
1998;30(1):41-45
- CountryRepublic of Korea
- Language:English
-
Abstract:
Oxidized low density lipoprotein (LDL) seems to take a part in atherogenesis through direct interactions with macrophages, endothelial cells, and smooth muscle cells, and is thought to participate in renal glomerular injury. For the purpose of illustrating the role of oxidized LDL in the human diseases, monoclonal antibodies were developed and characterized, recognizing oxidized LDL-specific epitopes that do not exist on native LDL. LDL was oxidized by the incubation with CuSO4, and used as immunogen. Splenocytes from the immunized mouse and mouse myeloma cells were fused to produce hybridomas, which were screened for the secretion of oxidized LDL-specific antibodies. Immunoblot analysis and binding affinity assay showed that these monoclonal antibodies recognize malondialdehyde-conjugated peptide epitopes.
