Effect of radiation dose variation on expression of caspase-3 in rat submandibular glands.
- Author:
Ki Jeong KWON
1
;
Yong Suk CHOI
;
Eui Hwan HWANG
;
Sang Rae LEE
;
Kwang Joon KOH
Author Information
1. Department of Oral and Maxillofacial Radiology, College of Dentistry, Kyung Hee University. hehan@khu.ac.kr
- Publication Type:Original Article
- Keywords:
Irradiation Dose;
Submandibular Gland;
Caspase-3
- MeSH:
Acinar Cells;
Animals;
Apoptosis;
Caspase 3*;
Head;
Humans;
Male;
Neck;
Necrosis;
Rats*;
Rats, Sprague-Dawley;
Submandibular Gland*
- From:Korean Journal of Oral and Maxillofacial Radiology
2006;36(1):7-15
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the caspase-3 expression in the acinar and ductal cells of rat submandibular glands after the irradiation of various doses. MATERIALS AND METHODS: The male Sprague-Dawley rats weighing approximately 250 gm were used for this study. The experimental group was irradiated with a single absorbed dose of 2, 5, 10, and 15 Gy on the head and neck region. The rats were sacrificed on the 1st, 3rd, 7th, 14th, 21st, and 28th day after irradiation. The specimens including the submandibular gland were sectioned and observed using histopathological and immunohistochemical methods. RESULTS: The local destruction of the acinar and ductal cells and the karyopyknotic nuclei of the acinar cells were observed in the 2 Gy and 5 Gy irradiation groups later than in the 10 Gy and 15 Gy irradiation groups. And the expression of caspase-3 was prominent only in the ductal cells in the 2 Gy and 5 Gy irradiation groups. CONCLUSION: This experiment suggests that radiation-induced apoptosis in the ductal cells of rat submandibular glands was induced by a low dose radiation associated with the activation of caspase-3 and radiation-induced necrosis was induced by a high dose radiation.
