B cell-associated immune profiles in patients with end-stage renal disease (ESRD).
- Author:
Kyoung Woon KIM
1
;
Byung Ha CHUNG
;
Eun Joo JEON
;
Bo Mi KIM
;
Bum Soon CHOI
;
Cheol Whee PARK
;
Yong Soo KIM
;
Seok Goo CHO
;
Mi La CHO
;
Chul Woo YANG
Author Information
1. Conversant Research Consortium in Immunologic Disease, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul 137-040, Korea. yangch@catholic.ac.kr, sjnam@skku.edu
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
B-lymphocyte subsets;
kidney failure, chronic;
precursor cells, B-lymphoid;
renal dialysis
- MeSH:
Adaptor Proteins, Signal Transducing/genetics;
Adult;
Antigens, CD19/metabolism;
B-Lymphocyte Subsets/immunology/metabolism;
B-Lymphocytes/*immunology/metabolism;
Cytokines/biosynthesis;
Female;
Humans;
Immunophenotyping;
Interleukin-10/metabolism;
Kidney Failure, Chronic/*immunology/metabolism;
Leukocytes, Mononuclear/metabolism;
Male;
Middle Aged;
Proto-Oncogene Proteins/genetics;
T-Lymphocytes, Regulatory/immunology/metabolism
- From:Experimental & Molecular Medicine
2012;44(8):465-472
- CountryRepublic of Korea
- Language:English
-
Abstract:
Most of the previous studies on immune dysregulation in end-stage renal disease (ESRD) have focused on T cell immunity. We investigated B cell subpopulations in ESRD patients and the effect of hemodialysis (HD) on B cell-associated immune profiles in these patients. Forty-four ESRD [maintenance HD patients (n = 27) and pre-dialysis patients (n = 17)] and 27 healthy volunteers were included in this study. We determined the percentage of B cell subtypes, such as mature and immature B cells, memory B cells, and interleukin (IL)-10+ cells, as well as B cell-producing cytokines (IL-10, IL-4 and IL-21) by florescent activated cell sorting (FACS). B cell-associated gene expression was examined using real-time PCR and B cell producing cytokines (IL-10, IL-4 and IL-21) were determined using an enzyme-linked immunosorbent assay (ELISA). The percentage of total B cells and mature B cells did not differ significantly among the three groups. The percentages of memory B cells were significantly higher in the pre-dialysis group than in the HD group (P < 0.01), but the percentage of immature B cells was significantly lower in the pre-dialysis group than in the other groups. The percentages of IL-10-expressing cells that were CD19+ or immature B cells did not differ significantly (P > 0.05) between the two subgroups within the ESRD group, but the serum IL-10 concentration was significantly lower in the pre-dialysis group (P < 0.01). The results of this study demonstrate significantly altered B cell-associated immunity. Specifically, an imbalance of immature and memory B cells in ESRD patients was observed, with this finding predominating in pre-dialysis patients.
