Beneficial Effect of Lespedeza cuneata (G. Don) Water Extract on Streptozotocin-induced Type 1 Diabetes and Cytokine-induced Beta-cell Damage.
10.20307/nps.2016.22.3.175
- Author:
Min Suk KIM
1
;
Bhesh Raj SHARMA
;
Dong Young RHYU
Author Information
1. Department of Oriental Medicine Resources and Institute of Korean Medicine Industry, Mokpo National University, Jeonnam 534-729, Republic of Korea. rhyudy@mokpo.ac.kr
- Publication Type:Original Article
- Keywords:
Cytokines;
Diabetes;
Lesepdeza cuneata;
Pancreatic beta cells;
Streptozotocin
- MeSH:
Animals;
Biomarkers;
Blood Glucose;
Blood Urea Nitrogen;
Body Weight;
Cytokines;
Diabetes Complications;
Fibronectins;
Glycosylation End Products, Advanced;
Insulin;
Insulin-Secreting Cells;
Insulinoma;
Lespedeza*;
Nitric Oxide;
Rats;
Streptozocin;
Thiobarbituric Acid Reactive Substances;
Water*
- From:Natural Product Sciences
2016;22(3):175-179
- CountryRepublic of Korea
- Language:English
-
Abstract:
The aim of this study was to evaluate the anti-diabetic effects of the water extract of Lespedeza cuneata (LCW) using rat insulinoma (RIN) m5F cells and streptozotocin (STZ)-induced diabetic rats. The effect of LCW on the protection of pancreatic beta cells was assessed using MTT assay, and nitric oxide production was assessed using Griess reagent. STZ-induced diabetic rats were treated with 100 and 400 mg/kg body weight of LCW for 5 weeks. In results, LCW significantly protected cytokine-induced toxicity and NO production, and increased insulin secretion in RINm5F cells. LCW significantly decreased serum blood glucose, thiobarbituric acid reactive substances (TBARS), blood urea nitrogen (BUN) and advanced glycation end products (AGEs) levels, and renal fibronectin expression in STZ-induced diabetic rats. Also, LCW effectively improved BW loss in STZ-induced diabetic rats. Thus, our results suggest that LCW has a beneficial effect on cytokine-induced pancreatic beta cell damage and biomarkers of diabetic complication in hyperglycemic rats.
