Effects of Gypenosides on Dopaminergic Neuronal Cell Death in 6-Hydroxydopamine-lesioned Rat Model of Parkinson's Disease with Long-term L-DOPA Treatment.
10.20307/nps.2016.22.3.187
- Author:
Keon Sung SHIN
1
;
Ting Ting ZHAO
;
Hyun Jin PARK
;
Kyung Sook KIM
;
Hyun Sook CHOI
;
Myung Koo LEE
Author Information
1. College of Pharmacy and Research Center for Bioresource and Health, Chungbuk National University, Cheongju, Chungbuk 28644, Korea. myklee@chungbuk.ac.kr
- Publication Type:Original Article
- Keywords:
Gypenosides;
6-Hydroxydopamine-lesioned rat;
Parkinson's disease;
Tyrosine hydroxylase immunohistochemistry;
L-DOPA
- MeSH:
3,4-Dihydroxyphenylacetic Acid;
Animals;
Cell Death*;
Dihydroxyphenylalanine;
Dopamine;
Dopaminergic Neurons*;
Homovanillic Acid;
Levodopa*;
Models, Animal*;
Norepinephrine;
Oxidopamine;
Parkinson Disease*;
Rats*;
Substantia Nigra;
Tyrosine 3-Monooxygenase
- From:Natural Product Sciences
2016;22(3):187-192
- CountryRepublic of Korea
- Language:English
-
Abstract:
The goal of this study was to determine whether gypenosides (GPS) exert protective effects against dopaminergic neuronal cell death in a 6-hydroxydopamine (OHDA)-lesioned rat model of Parkinson's disease (PD) with or without long-term 3,4-dihydroxyphenylalanine (L-DOPA) treatment. Rats were injected with 6-OHDA in the substantia nigra to induce PD-like symptoms; 14 days after injection, groups of 6-OHDA-lesioned animals were treated for 21 days with GPS (25 or 50 mg/kg) and/or L-DOPA (20 mg/kg). Dopaminergic neuronal cell death was assessed by counting tyrosine hydroxylase (TH)-immunopositive cells in the substantia nigra and measuring levels of dopamine, norepinephrine, 3,4-dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (HVA) in the striatum. Dopaminergic neuronal cell death induced by 6-OHDA lesions was ameliorated by GPS treatment (50 mg/kg). L-DOPA treatment exacerbated 6-OHDA-induced dopaminergic neuronal cell death; however, these effects were partially reversed by GPS treatment (25 and 50 mg/kg). These results suggest that GPS treatment is protective against dopaminergic neuronal cell death in a 6-OHDA-lesioned rat model of PD with long-term L-DOPA treatment. Therefore, GPS may be useful as a phytotherapeutic agent for the treatment of PD.
