Induction of Apoptosis with Kigelia africana fruits in HCT116 Human Colon Cancer Cells via MAPKs Signaling Pathway.
10.20307/nps.2016.22.3.209
- Author:
Tae Eun GUON
1
;
Ha Sook CHUNG
Author Information
1. College of Natural Sciences, Duksung Women's University, Seoul 01369, Republic of Korea. hasook@duksung.ac.kr
- Publication Type:Original Article
- Keywords:
Cytotoxicity;
MAPK signaling pathway;
Hallmarks of apoptosis
- MeSH:
Africa, Western;
Apoptosis*;
Bandages;
Caspase 3;
Caspase 9;
Cell Line;
Cell Survival;
Colon*;
Colonic Neoplasms*;
Ethanol;
Female;
Flow Cytometry;
Flowers;
Fruit*;
Humans*;
Lactation;
Mothers;
Propidium;
Rheumatic Diseases;
Trees;
Ulcer;
Wounds and Injuries
- From:Natural Product Sciences
2016;22(3):209-215
- CountryRepublic of Korea
- Language:English
-
Abstract:
Kigelia africana (Lam.) Benth. (Bignoniaceae) is a flowering plants in South, Central and West Africa and commonly known as the sausage tree (Eng.); worsboom (Afr.); umVunguta, umFongothi (Zulu); Modukguhlu (North Sotho); Muvevha (Venda). The dried, powdered fruits are used as dressing for wounds and ulcers, haemorrhoids, rheumatism, purgative, skin-firming, lactation in breast-feeding mothers. The aim of this study is to investigate the cytotoxic and apoptotic potentials of 70% ethanolic extracts of Kigelia africana fruits in HCT116 human colon cancer cells. Treatment of Kigelia africana fruits with various concentrations resulted in a sequence of characteristic of apoptosis, including loss of cell viability and morphological changes. Flow cytometry analysis showed Kigelia africana fruits increased the sub-G1 phase (apoptosis) population. Apoptosis confirmed by annexin V-fluorescein isothiocyanate and propidium iodide double staining in HCT116 human colon cancer cell lines. Moreover, analysis of the mechanism indicated that Kigelia africana fruits showed an increased Bax and Bcl-2 expressions in a dose-dependent manner, resulting in activation of hallmarks of apoptotic events, caspase-3, caspase-9 and cleaved poly-ADP-ribose polymerase. This is the first report to demonstrate the cytotoxicity of Kigelia africana fruits on HCT116 human colon cancer cells.
