Anti-Helicobacter pylori Compounds from Polygonum cuspidatum.
10.20307/nps.2016.22.3.220
- Author:
Atif Ali Khan KHALIL
1
;
Woo Sung PARK
;
Hye Jin KIM
;
Kazi Marjahan AKTER
;
Mi Jeong AHN
Author Information
1. College of Pharmacy and Research Institute of Pharmaceutical Sciences, Gyeongsang National University, Jinju 52828, Korea. amj5812@gnu.ac.kr
- Publication Type:Original Article
- Keywords:
Polygonum cuspidatum;
Anti-Helicobacter pylori activity;
Emodin;
2-Methoxy-6-acetyl-7-methyljuglone
- MeSH:
Anthraquinones;
Chromatography;
Emodin;
Fallopia japonica*;
Mass Spectrometry;
Plants;
Polygonum*;
Quercetin;
Silica Gel;
Stilbenes
- From:Natural Product Sciences
2016;22(3):220-224
- CountryRepublic of Korea
- Language:English
-
Abstract:
Anti-Helicobacter pylori activity guided fractionation led to the isolation of five anthraquinones, two stilbenes and one naphthoquinone from the EtOAc fraction of Polygonum cuspidatum, using silica gel column chromatography, Sephadex-LH20, MPLC and recrystallization. The chemical structures were identified to be physcion (1), emodin (2), anthraglycoside B (3), trans-resveratrol (4), anthraglycoside A (5), polydatin (6), 2-methoxy-6-acetyl-7-methyljuglone (7) and citreorosein (8) by UV, ¹H-NMR, ¹³C-NMR and mass spectrometry. Anti-Helicobacter pylori activity including MIC values of each compound was evaluated. All of the isolates exhibited anti-H. pylori activity of which MIC values were lower than that of a positive control, quercetin. Compounds 2 and 7 showed potent growth inhibitory activity. Especially, a naphthoquinone, compound 7 displayed most potent antibacterial activity with MIC₅₀ value of 0.30 µM and MIC₉₀ value of 0.39 µM. Although anti-H. pylori activity of this plant was previously reported, this is the first report on that of compounds isolated from this species. From these findings, P. cuspidatum roots or its isolates may be useful for H. pylori infection and further study is needed to elucidate mechanism of action.
