Recombinant influenza viruses as delivery vectors for hepatis B virus epitopes.
- Author:
Jae Min SONG
1
;
Kwang Hee LEE
;
Baik Lin SEONG
Author Information
- Publication Type:Original Article
- Keywords: NA stalk; Chimeric virus; Dual vaccine
- MeSH: Amino Acid Sequence; Enzyme-Linked Immunosorbent Assay; Epitopes; Epitopes, B-Lymphocyte; Helper Viruses; Hepatitis B virus; Herpesvirus 1, Cercopithecine; Influenza, Human; Kidney; Kinetics; Madin Darby Canine Kidney Cells; Neuraminidase; Orthomyxoviridae; Peptides; Viruses
- From:Clinical and Experimental Vaccine Research 2012;1(1):77-82
- CountryRepublic of Korea
- Language:English
- Abstract: PURPOSE: Neuraminidase (NA) of influenza virus contains stalk region that shows a great deal of variability in both amino acid sequence and length. In this paper, we investigated generation of recombinant influenza viruses that had hepatitis B virus (HBV) B cell epitopes in the NA stalk region as a dual vaccine candidate. MATERIALS AND METHODS: We used the WSH-HK reassortant helper virus for rescue of recombinant influenza virus containing HBV epitopes and reverse genetic protocol based on the use of micrococcal nuclease-treated virus cores for reconstitution of ribonucleoproteins. RESULTS: We successfully generated a chimeric influenza viruses which contained 22 amino acid peptides in the stalk region derived from the surface and pre-surface protein HBV. The growth kinetics of the recombinant viruses was investigated after infection of Madin-Darby canine kidney (MDCK) and Madin-Darby bovine kidney (MDBK) cells and the rIV-BVPreS virus showed higher titer than other viruses in MDCK cells. We also confirmed the presence of HBV epitopes in the chimeric viruses by enzyme-linked immunosorbent assay (ELISA) using anti-HBV polyclonal antibody. When the ratio of recombinant virus verse wild type virus was calculated by ELISA, recombinant viruses exhibited 2 fold higher values than the wild type virus. CONCLUSION: These results suggest that chimeric influenza virus which contained foreign antigens can be used as dual vaccine against both HBV and influenza viruses.
