GFP-tagged E. coli shows bacterial distribution in mouse organs: pathogen tracking using fluorescence signal.
- Author:
Pil Gu PARK
1
;
Min Hee CHO
;
Gi Eun RHIE
;
Haeseul JEONG
;
Hyewon YOUN
;
Kee Jong HONG
Author Information
- Publication Type:Original Article
- Keywords: Molecular imaging; GFP-tagged; Bacterial infections; Pathogen tracking; Escherichia coli
- MeSH: Animals; Bacterial Infections; Escherichia coli; Fluorescence; Intestines; Mice; Molecular Imaging; Sprains and Strains; Track and Field
- From:Clinical and Experimental Vaccine Research 2012;1(1):83-87
- CountryRepublic of Korea
- Language:English
- Abstract: PURPOSE: In vaccine efficacy evaluation, visualization of pathogens in whole organism at each time point would be able to reduce the consuming animals and provide the in vivo information within consistent background with identical organism. MATERIALS AND METHODS: Using IVIS spectrum whole live-animal imaging system, fluorescent intensity was optimized and visualized proportionately by concentrating Escherichia coli MC1061 strain which expresses GFP (E. coli-GFP) in BALB/C mice after injection. RESULTS: Local distribution of disseminated E. coli-GFP was traced in each organ by fluorescence. Detached organ showed more obvious fluorescent signal, and intestine showed strongest fluorescent signal. CONCLUSION: This in vivo imaging method using GFP-tagged pathogen strain suggest quantified infected pathogens by fluorescence intensity in whole animals can provide the information about the localization and distribution after infection.
