Effects of the Immunoglobulins of Amyotrophic Lateral Sclerosis on Intracellular Calcium in PC12 Cells.
- Author:
Sung Hun KIM
1
;
Sung Jun JUNG
;
Kyung Seok PARK
;
Manho KIM
;
Jun KIM
;
Hyun Jung KIM
;
Kwang Woo LEE
Author Information
1. Department of Neurology, College of Medicine, Seoul National University.
- Publication Type:Original Article
- Keywords:
Amyotrophic lateral sclerosis;
Calcium;
Immunoglobulins
- MeSH:
Amyotrophic Lateral Sclerosis*;
Animals;
Calcium Channels;
Calcium Channels, L-Type;
Calcium*;
Fluorometry;
Fura-2;
Humans;
Immunoglobulins*;
Motor Neurons;
Neurons;
Nicardipine;
Oxidative Stress;
PC12 Cells*;
Potassium Chloride
- From:Journal of the Korean Neurological Association
2000;18(6):728-734
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The motor neuronal death in amyotrophic lateral sclerosis (ALS) arise from several mechanisms including excitotoxicity, oxidative stress, immunologic or calcium dysregulation. Though the immunoglobulin mediated neuronal death has been implicated through changes of calcium influx, the direct evidence showing the increase of intracellular calcium level is rare. The purpose of our study is to directly measure the calcium in cultured neurons treated with immunoglobulins from ALS patients to further understand the role of calcium in motor neuron degeneration. METHODS: Immunoglobins purified from serum in patients with ALS (n=8) and in control (n=8) were incubated for 24 hours in PC12 cell line. The intracellular calcium level was measured by fluorometry using calcium chelating dye, fura-2. High concentration of potassium chloride were used to evoke calcium influx, and L-type calcium channel current were measured using nicardipine, the L-type specific calcium channel blocker. RESULTS: Basal level of intracellular calcium was higher in the cells treated with immunoglobulins from ALS. However, the calcium increase evoked by 30 mM or 140 mM potassium chloride were lower in ALS than in control. The calcium increase through L-type calcium channel in ALS group was not significantly different from control. Conclusions ; Considering the proposed role of the calcium in neuronal degeneration, present data suggest increased intracellular calcium is important in neurodegenerative mechanism caused by ALS immunoglobulins.
