The Effects of Scavengers on Reactive Oxygen Species in Protection of Ultraviolet A Induced Damage in langerhans Cells in the Skin.
- Author:
Soo Min KIM
;
Hyung Joo KIM
;
Seung Kyung HANN
;
Yoon Kee PARK
- Publication Type:Original Article
- Keywords:
Ultraviolet A;
Reactive Oxygen Species;
Scavengers;
Lanigerhans Cell
- MeSH:
Animals;
Catalase;
Ear;
Hydrogen;
Langerhans Cells*;
Mice;
Mice, Inbred C3H;
Oxygen;
Reactive Oxygen Species*;
Skin*;
Sodium;
Sodium Azide;
Superoxides
- From:Korean Journal of Dermatology
1994;32(3):383-390
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: It is reported by rnany t,hat UVB and large coses of UVA damage epidermal Langerhans cells. UVA irradiated skin produces reactive oxygen soecies such as superoxide anion(O ), hydrogen peroxide(H O), hydroxyl radical( OH) and singlet oxigen(O ). Reactive oxygen species such as O are invnlved in the process of decreasing the number if epidermal Langerhans cells after irradiation with UVB. However, no well established studies have been carried out after irradiation with UVA. OBJECTIVES: We wished to find out whether the UVA-induced decrease in the number of Ia Langerhans cells would be protected by scavengers of reactive oxiygen species. METHODS: We irradiated 200J/cm and 400J/cm of UVA on thi ears of C3H mice with or without intravenous injectior. of SOD, catalase, and sodium azide which are the scavengers of O , HO and O, respectively. We biopsied the ears of mice 2 days after irradiaion, stained them with immunope-roxidase technique, and counted the number of Langerhans cells. RESULTS: 1, There was significant decrease in the number of Ia Langerhans cells in the 200J/cm or 400J/cm of UVA irradiated group compared to the normal control group. 2. The pretreatment groups with SOD, catalase, and sodium to before irradiation with 200J/cm or 400J/cm of UVA showed less decrease in the number of la Langerhans cells compared to the 200J/cm or 400J/cm irradiated group with UVA only. CONCLUSION: Frorn the above result, we can deduce that reactive oxygen species are involved in the decrease in the number of Ia Langerhans cells induced by 200J/cm. or 400J/cm of UVA irradiation.
