Osteoblast differentiation of human bone marrow stromal cells (hBMSC) according to age for bone tissue engineering.
10.5125/jkaoms.2010.36.4.243
- Author:
Gin Ah SONG
1
;
Hyun Mo RYOO
;
Jin Young CHOI
Author Information
1. Department of Molecular Genetics, School of Dentistry and Dental Research Institute, BK21 Program, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Mesenchymal stem cells;
Osteoblast differentiation;
Histone deacetylase inhibitors;
DNA methyltransferase inhibitors;
Aging
- MeSH:
Adult;
Aging;
Bone and Bones;
Bone Marrow;
Chromatin Assembly and Disassembly;
Durapatite;
Histone Deacetylase Inhibitors;
Humans;
Hydroxamic Acids;
Inflammation;
Mesenchymal Stromal Cells;
Osteoblasts;
Tissue Donors;
Transplants
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2010;36(4):243-249
- CountryRepublic of Korea
- Language:English
-
Abstract:
Tissue engineered bone (TEB) can replace an autogenous bone graft requiring an secondary operation site as well as avoid complications like inflammation or infection from xenogenic or synthetic bone graft. Adult mesenchymal stem cells (MSC) for TEB are considered to have various ranges of differentiation capacity or multipotency by the donor site and age. This study examined the effect of age on proliferation capacity, differentiation capacity and bone morphogenetic protein-2 (BMP-2) responsiveness of human bone marrow stromal cells (hBMSC) according to the age. In addition, to evaluate the effect on enhancement for osteoblast differentiation, the hBMSC were treated with Trichostatin A (TSA) and 5-Azacitidine (5-AZC) which was HDAC inhibitors and methyltransferase inhibitors respectively affecting chromatin remodeling temporarily and reversibly. The young and old group of hBMSC obtained from the iliac crest from total 9 healthy patients, showed similar proliferation capacity. Cell surface markers such as CD34, CD45, CD90 and CD105 showed uniform expression regardless of age. However, the young group showed more prominent transdifferentiation capacity with adipogenic differentiation. The osteoblast differentiation capacity or BMP responsiveness was low and similar between young and old group. TSA and 5-AZC showed potential for enhancing the BMP effect on osteoblast differentiation by increasing the expression level of osteogenic master gene, such as DLX5, ALP. More study will be needed to determine the positive effect of the reversible function of HDAC inhibitors or methyltransferase inhibitors on enhancing the low osteoblast differentiation capacity of hBMSC.
