Tissue-specific expression of the NOD-like receptor protein 3 in BALB/c mice.
10.4142/jvs.2014.15.2.173
- Author:
Zhiqiang HUANG
1
;
Meng YU
;
Shuang TONG
;
Kun JIA
;
Rongchang LIU
;
Heng WANG
;
Shoujun LI
;
Zhangyong NING
Author Information
1. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. shoujunli@scau.edu.cn
- Publication Type:Original Article
- Keywords:
BALB/c mice;
gene expression profile;
NLRP3;
tissue distribution
- MeSH:
Animals;
Carrier Proteins/*genetics/metabolism;
*Gene Expression Regulation;
*Immunity, Innate;
Mice;
Mice, Inbred BALB C;
Molecular Sequence Data;
Organ Specificity;
RNA, Messenger/genetics/metabolism;
Real-Time Polymerase Chain Reaction;
Sequence Analysis, DNA
- From:Journal of Veterinary Science
2014;15(2):173-177
- CountryRepublic of Korea
- Language:English
-
Abstract:
Activation of the innate immune system requires recognition of pathogen-associated molecular patterns, such as NOD-like receptors. The NOD-like receptor protein 3 (NLRP3) inflammasome is involved in induction of the pro-inflammatory cytokine, IL-1beta, and subsequent inflammatory responses. NLRP3 inflammasome plays important roles in the inflammatory and innate immune responses associated with autoimmune/inflammatory syndrome. However, analysis of the tissue distribution and expression profiles in BALB/c mice is still incomplete. In this study, we investigated the tissue distribution and expression pattern of NLRP3 in BALB/c mice to further elucidate its function in innate immunity in this commonly used laboratory animal model. NLRP3 mRNA expression levels and tissue distribution of the protein were investigated by real-time quantitative PCR and immunohistochemical analyses, respectively. NLRP3 mRNA expression was higher in the kidney and inguinal lymph nodes than in other tissues. Cytoplasmic expression of NLRP3 was detected in the epithelial reticular cells of the spleen and thymus, lymphocytes in the inguinal lymph nodes, cardiac muscle cells, cerebral cortex neurons, alveolar macrophages, renal tubule cells and liver sinusoidal endothelial cells. The results of this study will assist investigators in interpreting site-specific functions and roles of NLRP3 in inflammatory responses.
