Effect of sonicated extracts of Enterococcus faecalis on the production of matrix metalloproteinase-8 by human polymorphonuclear neutrophils.
10.5395/JKACD.2005.30.2.138
- Author:
Sung Kyung PARK
1
;
Won Jun SHON
;
Sung Sam LIM
Author Information
1. Department of Conservative Dentistry, College of Dentistry, Seoul National University, Korea. limss@snu.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
E. faecalis;
MMP-8;
Calcium hydroxide;
PMN leukocyte
- MeSH:
Calcium Hydroxide;
Enterococcus faecalis*;
Enterococcus*;
Enzyme-Linked Immunosorbent Assay;
Humans*;
Lipopolysaccharides;
Matrix Metalloproteinase 8*;
Neutrophils*
- From:Journal of Korean Academy of Conservative Dentistry
2005;30(2):138-144
- CountryRepublic of Korea
- Language:English
-
Abstract:
This in vitro study monitored MMP-8 production on PMN by stimulated with the following three groups; Sonicated extracts of E. faecalis (SEF), SEF treated with Ca(OH)2 (12.5mg/ml) for 7 days, and lipopolysaccharides (LPS) of E. coli. The level of MMP-8 in each group was immediately measured by ELISA. The data were analyzed with Kruskal-Wallis test and Mann-Whitney U test. In the SEF group, the level of production of MMP-8 was higher than the negative control group in low concentration (0.05microg/ml) of SEF (p < 0.05), but it decreased with an increase in the concentration of SEF (p < 0.05). In the case of SEF treated with Ca(OH)2, all of the MMP levels were higher than negative control group (p < 0.05), but no statistical difference was found among the different SEF concentrations (p > 0.05). All of the levels in E. coli LPS were increased with increasing concentrations (p < 0.05). According to this study we could summarize as follows: 1. MMP-8 was expressed at low level in untreated PMN group and the levels of MMP-8 were upregulated in PMN stimulated by E. coli LPS groups. 2. In the SEF groups, the level of production of MMP-8 decreased with an increase in the concentration of SEF (p < 0.05). So E. faecalis may have suppressive effect on the production of MMP-8 by PMN. 3. In the case of SEF treated with Ca(OH)2, all of the MMP levels at different SEF concentrations were higher than untreated PMN group (p < 0.05), but no statistical difference was found among the different SEF concentrations (p > 0.05).
