Anisakis pegreffii Larvae in Sea Eels (Astroconger myriaster) from the South Sea, Republic of Korea.
10.3347/kjp.2015.53.3.349
- Author:
Jaeeun CHO
1
;
Hyemi LIM
;
Bong Kwang JUNG
;
Eun Hee SHIN
;
Jong Yil CHAI
Author Information
1. Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea. cjy@snu.ac.kr
- Publication Type:Brief Communication
- Keywords:
Anisakis pegreffii;
sea eel;
PCR-RFLP;
ITS1;
cox2
- MeSH:
Animals;
Anisakiasis/parasitology/*veterinary;
Anisakis/classification/genetics/*isolation & purification;
DNA, Helminth/genetics;
DNA, Ribosomal Spacer/genetics;
*Eels/growth & development;
Fish Diseases/*parasitology;
Larva/classification/genetics;
Phylogeny;
Polymorphism, Restriction Fragment Length;
Republic of Korea
- From:The Korean Journal of Parasitology
2015;53(3):349-353
- CountryRepublic of Korea
- Language:English
-
Abstract:
Anisakis simplex sensu stricto (s.s.), Anisakis pegreffii, Anisakis berlandi (=A. simplex sp. C), and Anisakis typica are the 4 major species of Anisakis type I larvae. In the Republic of Korea (Korea), A. pegreffii, A. berlandi, and A. typica larvae in fish hosts has seldom been documented. In this study, molecular analysis was performed on Anisakis larvae from the sea eels (Astroconger myriaster), the major source of human anisakiasis in Korea, collected from Tongyeong City, a southern coastal area of Korea. All 20 sea eels examined were infected with Anisakis type I larvae (160 larvae; 8 per fish). Their species were analyzed using PCR-RFLP patterns and nucleotide sequences of internal transcribed spacers (ITS1, 5.8 subunit gene, and ITS2) and mitochondrial cytochrome c oxidase 2 (cox2). Most (86.8%; 112/129) of the Anisakis type I larvae were A. pegreffii, and 7.8% (10/129) were A. typica. The remaining 5.4% (7/129) was not identified. Thus, A. pegreffii is the major species of anisakid larvae in sea eels of the southern coast of Korea.
