Prostaglandin E2 stimulates angiogenesis by activating the nitric oxide/cGMP pathway in human umbilical vein endothelial cells.
- Author:
Seung NAMKOONG
1
;
Seon Jin LEE
;
Chun Ki KIM
;
Young Mi KIM
;
Hun Taeg CHUNG
;
Hansoo LEE
;
Jeong A HAN
;
Kwon Soo HA
;
Young Guen KWON
;
Young Myeong KIM
Author Information
1. Vascular System Research Center, School of Medicine, Kangwon National University, Chuncheon 200-701, Korea. ymkim@kangwon.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
cyclic AMP;
endothelial cells;
endothelial nitric oxide synthase;
prostaglandin E2
- MeSH:
1-Phosphatidylinositol 3-Kinase/antagonists & inhibitors;
Animals;
Aorta;
Cell Movement/drug effects;
Cell Proliferation/drug effects;
Cyclic AMP/metabolism/pharmacology;
Cyclic GMP/biosynthesis/*metabolism;
Dinoprostone/*pharmacology;
Endothelial Cells/*drug effects/metabolism;
Enzyme Inhibitors/pharmacology;
Humans;
Mice;
Mice, Knockout;
Neovascularization, Physiologic/*drug effects;
Nitric Oxide/biosynthesis/*metabolism;
Nitric Oxide Synthase Type III/deficiency/metabolism;
Phosphorylation/drug effects;
Proto-Oncogene Proteins c-akt/metabolism;
Rats;
Rats, Sprague-Dawley;
Research Support, Non-U.S. Gov't;
Signal Transduction/*drug effects;
Umbilical Veins/cytology/*drug effects/metabolism
- From:Experimental & Molecular Medicine
2005;37(6):588-600
- CountryRepublic of Korea
- Language:English
-
Abstract:
Prostaglandin E2(PGE2), a major product of cyclooxygenase, has been implicated in modulating angiogenesis, vascular function, and inflammatory processes, but the underlying mechanism is not clearly elucidated. We here investigated the molecular mechanism by which PGE 2 regulates angiogenesis. Treatment of human umbilical vein endothelial cells (HUVEC) with PGE 2 increased angiogenesis. PGE 2 increased phosphorylation of Akt and endothelial nitric oxide synthase (eNOS), eNOS activity, and nitric oxide (NO) production by the activation of cAMP-dependent protein kinase (PKA) and phosphatidylinositol 3-kinase (PI3K). Dibutyryl cAMP (DB-cAMP) mimicked the role of PGE 2 in angiogenesis and the signaling pathway, suggesting that cAMP is a down-stream mediator of PGE 2. Furthermore, PGE 2 increased endothelial cell sprouting from normal murine aortic segments, but not from eNOS-deficient ones, on Matrigel. The angiogenic effects of PGE 2 were inhibited by the inhibitors of PKA, PI3K, eNOS, and soluble guanylate cyclase, but not by phospholipase C inhibitor. These results clearly show that PGE 2 increased angiogenesis by activating the NO/cGMP signaling pathway through PKA/PI3K/Akt-dependent increase in eNOS activity.
