Transcription of class II MHC gene by interferon-gamma in FRTL-5 cells.
10.3346/jkms.1992.7.3.189
- Author:
Myung Shik LEE
1
;
Jhin Oh LEE
;
Tae Woong KANG
;
Je Ho LEE
Author Information
1. Department of Internal Medicine, Korea Cancer Center Hospital, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
interferon-gamma;
class II MHC;
transcription;
cycloheximide
- MeSH:
Animals;
Cell Line;
Cycloheximide/pharmacology;
Gene Expression Regulation/drug effects;
*Genes, MHC Class II;
Interferon-gamma/*pharmacology;
Protein Biosynthesis/drug effects;
Rats;
Transcription, Genetic/*drug effects
- From:Journal of Korean Medical Science
1992;7(3):189-192
- CountryRepublic of Korea
- Language:English
-
Abstract:
The intracellular mechanism by which interferon-gamma induces the expression of class II major histocompatibility complex (MHC) antigen in nonlymphoid cells is not clear. The effect of recombinant rat interferon-gamma (IFN-gamma), and cycloheximide on the expression of class II MHC gene was studied using the techniques of immunocytochemical staining and northern blot analysis. IFN-gamma induced de novo transcription of class II MHC gene and class II MHC antigen expression on the cell surface. Cycloheximide did not inhibit IFN-gamma-induced class II MHC antigen expression in a dose-dependent manner indicating translational blockade. These results suggest that IFN-gamma induces class II MHC antigen expression via de novo transcription of class II MHC gene leading to synthesis of new class II MHC molecule.
