Retinal Protective Effects of Minocycline via Anti-apoptosis on Oxygen-induced Retinopathy in Neonatal Rats.
- Author:
Yoon Young JANG
1
;
Eok Soo SUH
;
Woo Taek KIM
Author Information
1. Department of Pediatrics, School of Medicine, Catholic University of Daegu, Daegu, Korea. wootykim@cu.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Retinopathy of Prematurity;
Minocycline;
Apoptosis;
Bcl-2;
Bax;
caspase-3
- MeSH:
Animals;
Antibodies;
Apoptosis;
Blindness;
Blotting, Western;
Caspase 3;
Cell Culture Techniques;
Diterpenes;
Fluorescent Antibody Technique;
Humans;
Hyperoxia;
Infant, Newborn;
Infant, Premature;
Minocycline;
Models, Animal;
Oxygen;
Rats;
Real-Time Polymerase Chain Reaction;
Retinal Detachment;
Retinaldehyde;
Retinopathy of Prematurity;
RNA, Messenger;
Survival Rate
- From:Korean Journal of Perinatology
2010;21(1):26-39
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Retinopathy of prematurity (ROP) is a leading cause of blindness with retinal detachment due to oxygen toxicity in preterm infants. Recently advances in neonatal care had to led improved survival rates in premature infants and ROP re-emerged as a significant clinical problem. In the present study, we aimed to determine the protective abilities of minocycline in a animal model of ROP and a primary retinal cell cultures of neonatal rat via anti-apoptotic actions using Western blotting and real-time PCR with Bcl-2, Bax and caspase-3 antibodies and mRNAs. METHODS: In the in vivo oxygen-induced retinopathy (OIR), the cyclic hyperoxia was performed that 80% O2 for 1 day and 21% O2 for 1 day from P1-14 of newborn rats. Minocycline was injected intravitreously for 7 days and sacrificed at P21. In the in vitro OIR, primary retinal cell culture was done using P0-P2 SD rats. Hyperoxia injury was done for 100% O2 exposure for 6 hours. Western blotting and real-time PCR using Bcl-2, Bax and caspase-3 antibody and primer were done in the rat model of ROP and the dispersed retinal cell culture. To identify photoreceptors of retinal cells the immunofluorescence assay photoreceptor marker, IRBP, was used. RESULTS: In the in vivo OIR, the expression of Bcl-2 antibody and mRNA was increased and those of Bax and caspase-3 were reduced in the minocycline-treated group. In the in vitro OIR, the result was the same as above. CONCLUSION: In conclusion, minocycline was suggested to have retinal protective effects for hyperoxic injury via anti-apoptotic mechanism.
