Gene Therapy Using Hepatocyte Growth Factor Expressing Adenovirus Improves Skin Flap Survival in a Rat Model.
10.3346/jkms.2014.29.S3.S228
- Author:
Dong Kyun RAH
1
;
In Sik YUN
;
Chae Ok YUN
;
Sae Bin LEE
;
Won Jai LEE
Author Information
1. Institute for Human Tissue Restoration, Department of Plastic & Reconstructive Surgery, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea. pswjlee@yuhs.ac
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Hepatocyte Growth Factor;
Adenovirus;
Gene Therapy;
Skin Flap
- MeSH:
Adenoviridae/genetics;
Animals;
Genetic Therapy/*methods;
Graft Survival/genetics;
Hepatocyte Growth Factor/biosynthesis/*genetics;
Male;
Models, Animal;
Neovascularization, Physiologic/*genetics;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Reconstructive Surgical Procedures;
Skin Transplantation/*methods;
Surgical Flaps/*surgery
- From:Journal of Korean Medical Science
2014;29(Suppl 3):S228-S236
- CountryRepublic of Korea
- Language:English
-
Abstract:
Hepatocyte growth factor (HGF) is a potent angiogenic factor that can stimulate the production of blood vessels in ischemic tissue. We investigated whether gene therapy using HGF-expressing adenovirus could enhance skin flap survival. Sprague-Dawley rats were randomly divided into three groups. Rats were subdermally injected with HGF-expressing adenovirus (HGF virus group), recombinant HGF (rhHGF group), or phosphate buffered saline (PBS group) 2 days before and immediately after 3 x 9 cm caudal flap elevation. The survival area of the skin flap, the ratio of blood flow, CD31-positive vessels and, VEGF expression were examined. Skin flap viability was significantly increased in the HGF virus group compared to the rhHGF and PBS groups (71.4% +/- 5.9%, 63.8%+/- 6.4%, and 39.2% +/- 13.0%, respectively) (P = 0.025). Furthermore, the blood flow ratio was significantly increased in the HGF virus group. In the HGF virus group, the number of CD31-positive vessels and vascular endothelial growth factor (VEGF) expression were significantly increased. Gene therapy using HGF-expressing adenovirus increase VEGF expression, the number of viable capillaries, and blood flow to the flap, thereby improving skin flap survival.
