Chronological Changes in the Rotational Behavior in Response to Apomorphine Administration in 6-Hydroxydopamine Parkinsonian Rat.
- Author:
Keun Sik HONG
1
;
Byung Gon KIM
;
Beom Seok JEON
;
Byung Woo YOON
;
Kwang Woo LEE
;
Jae Kyu ROH
;
Sang Bok LEE
;
Ho Jin MYUNG
Author Information
1. Department of Neurology, College of Medicine, Seoul National University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Rotational behavior;
Apomorphine
- MeSH:
Animals;
Apomorphine*;
Dopaminergic Neurons;
Neuroprotective Agents;
Oxidopamine*;
Rats*;
Substantia Nigra;
Transplants
- From:Journal of the Korean Neurological Association
1999;17(1):117-121
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Apomorphine-induced rotational behavior of unilateral 6-hydroxydopamine (OHDA) lesioned rat is widely used to develop anti-Parkinsonian treatments including drugs, neuroprotective therapy, and neural graft. Time course of changes in rotational behavior after lesioning, however, has not been fully elucidated. The aim of this study was to observe the chronological changes in the rotational response and to find the optimal period when this model is used for investigation of various therapies. METHODS: 6-OHDA was stereotaxically delivered to the unilateral substantia nigra in 13 rats. Rotational responses to apomorphine administrations were counted in the rotomotor on 2, 4, 8, 12, and 14 weeks after lesioning. RESULTS: The total turns for two hours increased continuously up to eight weeks, and then plateaued. CONCLUSIONS: Apomorphine-induced rotations increase until eight weeks after 6-OHDA lesioning. Therefore, this Parkinsonian model should be used at least eight weeks after lesioning. Even though priming was not excluded as an explanation in the experiment, we reason that progressive degeneration of dopaminergic neurons may explain the chronological changes in rotational behavior.
