Expression of extracellular martix genes in scleroderma fibroblast cultures.
- Author:
Kyu Suk LEE
;
Seok Ju KIM
;
Min Ho SUH
;
Joon Young SONG
- Publication Type:Original Article
- Keywords:
Extracellular matrix genes;
Scleroderma;
Northern and slot-blot hybridization
- MeSH:
Blotting, Northern;
Cell Line;
Collagen;
Connective Tissue Diseases;
Extracellular Matrix;
Fibroblasts*;
Procollagen;
RNA, Messenger;
Skin
- From:Korean Journal of Dermatology
1991;29(3):322-330
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Scleroderma is a connective tissue disease characterized by excessive accumulation of collagen in skin and visceral organs due to increased collagen production by scleroderma fibroblasts. The basic etiology of this collagen accumulation is not known. We examined the expression of various extracellular matrix genes in cultured fibrolasts using Northern blot and slot-blot hybridization. The scleroderma fibroblasts exhibited characteristic mRNA size of extracellular matrix genes and prominanty increased type I and III procollagen mRNAs levels compared to control fibroblasts cultures from univolved skin. The ratios of type I /IE procollagen in scleroderma cell lines were not so much different to the controls. These results indicate that increases of collagen biosynthesis in scleroderma can be a accounted for, at least in part, by an increased content of transcriptable type I and type JE procollagen mRNAs, both.
