The activity of antimelanocyte autoantibodies in vitiligo patients.
- Author:
Yoon Kee PARK
;
Seung Kyung HANN
;
Min Seok SONG
;
Jung Ku YOON
;
Hyung Il KIM
- Publication Type:In Vitro ; Original Article
- Keywords:
Vitiligo;
Autoantibody;
Complement mediated cytotoxicity
- MeSH:
Acridine Orange;
Antibodies;
Autoantibodies*;
Complement Activation;
Ethidium;
Humans;
Melanocytes;
PUVA Therapy;
Vitiligo*
- From:Korean Journal of Dermatology
1991;29(3):391-398
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Several observation suggest that the antimelanocyte autoantibodies could play a role in melanocyte destruction. Some experiments indicate that melanocyte antibodies from patients with vitiligo can kill melanocyte in vitro. In these experiments, we demonstrated that vitiligo patient's sera containing antimelanocyte antibodies can lyse cultured human melanocytes by complement activation. Melanocyte cytotoxicity was measured using the ethidium bromide/ acridine orange viability assay. Significant melanocyte cytotoxicity was seen in sera from patients with both active and inactive vitiligo(p<0.01). Melanocyte cytotoxicity measured with complement-mediated cytotoxicity decreased after systemic steroid treatment(p<0.05) ; however melanocyte cytotoxicity showed no significant change with systemic PUVA therapy.
