Identification and Antibiotic Susceptibility of the Bacteria from Non-odontogenic Infectious Lesions.
- Author:
Yong Min KIM
1
;
Jae Jin KIM
;
Mija KIM
;
Soon Nang PARK
;
Hwa Sook KIM
;
Joong Ki KOOK
;
Hak Kyun KIM
Author Information
1. Department of Oral & Maxillofacial Surgery, Dream Dental Clinic, Daejeon, Korea.
- Publication Type:Original Article
- Keywords:
mucormycosis;
actinomycosis;
16S rDNA
- MeSH:
Acinetobacter calcoaceticus;
Actinomyces;
Actinomycosis;
Amoxicillin;
Anti-Bacterial Agents;
Bacillus subtilis;
Bacteria*;
Capnocytophaga;
Cefuroxime;
Clindamycin;
Enterobacter;
Enterococcus faecalis;
Erythromycin;
Genes, rRNA;
Humans;
Inflammation;
Klebsiella pneumoniae;
Microbial Sensitivity Tests;
Mucormycosis;
Neisseria;
Parotid Gland;
Penicillin G;
Penicillins;
Tetracycline;
Vancomycin;
Veillonella;
Wound Infection
- From:International Journal of Oral Biology
2014;39(2):87-95
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The purpose of this study was to isolate and identify bacteria from the 4 patients with non-odontogenic infectious lesions (mucormycosis, chronic inflammation from wound infection, and two actinomycosis) and determine their antimicrobial susceptibility against eight antibiotics. Bacterial culture was performed under three culture conditions (anaerobic, CO2, and aerobic incubator). The bacterial strains were identified by 16S rRNA gene (16S rDNA) sequence comparison analysis method. For investigating the antimicrobial susceptibility of the bacteria against eight antibiotics, penicillin G, amoxicillin, tetracycline, cefuroxime, erythromycin, clindamycin, vancomycin, and Augmentin(R) (amoxicillin + clavulanic acid), minimum inhibitory concentration (MIC) measurement was performed using broth microdilution assay. Nosocomial pathogens such as Enterococcus faecalis, Klebsiella pneumoniae, Bacillus subtilis, and Neisseria flavescens were isolated from mucormycosis. Veillonella parvula, Enterobacter hormaechei, and Acinetobacter calcoaceticus were isolated from chronic inflammatory lesion. Actinomyces massiliensis was isolated from actinomycosis in parotid gland. Capnocytophaga ochracea was isolated from actinomycosis in buccal region in anaerobic condition. There was no susceptible antibiotic to all bacteria in mucormycosis. Tetracycline was susceptible to all bacteria in chronic inflammation. C. ochracea was resistant to vancomycin and penicillin G; and other antibiotics showed susceptibility to all bacteria in actinomycosis. The results indicated that the combined treatment of two or more antibiotics is better than single antibiotic treatment in mucormycosis, and penicillin is the first recommended antibiotic to treat actinomycosis.
