Analyses of the TCR repertoire of MHC class II-restricted innate CD4+ T cells.
- Author:
Byung Hyun KANG
1
;
Hye Sook MIN
;
You Jeong LEE
;
Bomi CHOI
;
Eun Ji KIM
;
Jonghoon LEE
;
Jeong Rae KIM
;
Kwang Hyun CHO
;
Tae Jin KIM
;
Kyeong Cheon JUNG
;
Seong Hoe PARK
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- MeSH: Amino Acid Sequence; Animals; Antigens, Surface/metabolism; CD4-Positive T-Lymphocytes/cytology/*immunology/*metabolism; Cell Communication; Cell Differentiation/genetics/immunology; Clonal Evolution; Histocompatibility Antigens Class II/*immunology; *Immunity, Innate; Immunophenotyping; Lymphocyte Count; Mice; Mice, Knockout; Mice, Transgenic; Peptide Fragments/chemistry; Phenotype; Receptors, Antigen, T-Cell/chemistry/*genetics/metabolism; Receptors, Antigen, T-Cell, alpha-beta/chemistry/genetics; Spleen/cytology; Thymocytes/cytology/immunology/metabolism
- From:Experimental & Molecular Medicine 2015;47(3):e154-
- CountryRepublic of Korea
- Language:English
- Abstract: Analysis of the T-cell receptor (TCR) repertoire of innate CD4+ T cells selected by major histocompatibility complex (MHC) class II-dependent thymocyte-thymocyte (T-T) interaction (T-T CD4+ T cells) is essential for predicting the characteristics of the antigens that bind to these T cells and for distinguishing T-T CD4+ T cells from other types of innate T cells. Using the TCRmini Tg mouse model, we show that the repertoire of TCRalpha chains in T-T CD4+ T cells was extremely diverse, in contrast to the repertoires previously described for other types of innate T cells. The TCRalpha chain sequences significantly overlapped between T-T CD4+ T cells and conventional CD4+ T cells in the thymus and spleen. However, the diversity of the TCRalpha repertoire of T-T CD4+ T cells seemed to be restricted compared with that of conventional CD4+ T cells. Interestingly, the frequency of the parental OT-II TCRalpha chains was significantly reduced in the process of T-T interaction. This diverse and shifted repertoire in T-T CD4+ T cells has biological relevance in terms of defense against diverse pathogens and a possible regulatory role during peripheral T-T interaction.
