Activity of Matrix Metalloproteinase-2 and its Significance after Resection of Stage I Non-small Cell Lung Cancer.
- Author:
Sang Hui KIM
1
;
Young Sook HONG
;
Jinseon LEE
;
Dae Soon SON
;
Yu Sung LIM
;
In Seung SONG
;
Hye Sook LEE
;
Do Hun KIM
;
Jhingook KIM
;
Yong Soo CHOI
Author Information
1. Cancer Research Center, Center for Clinical Research, Samsung Biomedical Research Institute, Korea.
- Publication Type:Original Article
- Keywords:
Lung neoplasms;
Cell biology;
Neoplasm proteins;
Neoplasm outcomes
- MeSH:
Basement Membrane;
Carcinoma, Non-Small-Cell Lung*;
Collagen Type IV;
Drug Therapy;
Humans;
Lung Neoplasms;
Matrix Metalloproteinase 2*;
Neoplasm Metastasis;
Neoplasm Proteins;
Peptide Hydrolases;
Radiotherapy;
Recurrence
- From:The Korean Journal of Thoracic and Cardiovascular Surgery
2005;38(1):38-43
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Matrix metalloproteinase-2 (MMP-2) is a class of proteolytic enzymes that digest collagen type IV and other components of the basement membrane. It plays a key role in the local invasion and the formation of distant metastases by various malignant tumors. The aim of this study was to evaluate the activity of MMP-2 and its significance as a prognostic marker in resected stage I non-small cell lung cancer (NSCLC). MATERIAL AND METHOD: In this study we obtained fresh-frozen samples of tumor and non-tumor tissues from 34 patients with stage I NSCLC who underwent resection without preoperative radiotherapy or chemotherapy. After the extraction of total protein from tissue samples, MMP-2 activities were assessed by gelatin-substrate-zymography. The activities were divided into the higher or lower groups. RESULT: The MMP-2 activities were higher in tumor tissues than in non-tumor tissues. The MMP-2 activity of non-tumor tissues in recurrent group was higher than in non-recurrent group (p<0.01). Also the patients with higher MMP-2 activity of non-tumor tissues showed poor 5 year survival (p<0.01). CONCLUSION: This result indicates that the higher level of MMP-2 activity in the non-tumor tissue is associated with the recurrence and survival after the resection of stage I NSCLC. Therefore, MMP-2 activity in the non-tumor tissue could be used as a potential prognostic marker for the resected stage I-NSCLC.
