Classification of Biological Effect of 1,763 MHz Radiofrequency Radiation Based on Gene Expression Profiles.
- Author:
Chang Nim IM
1
;
Eun Hye KIM
;
Ae Kyung PARK
;
Woong Yang PARK
Author Information
1. Genomics Core Laboratory and Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul 110-799, Korea. wypark@snu.ac.kr
- Publication Type:Original Article
- Keywords:
biomarker;
heat shock;
quantitative RT-PCR;
radiofrequency radiation
- MeSH:
Absorption;
Cell Count;
Cellular Phone;
Fibroblast Growth Factor 2;
Fibroblasts;
Gene Expression;
Heat-Shock Proteins;
Hot Temperature;
Humans;
Lung;
Radiation, Ionizing;
Transcriptome;
Biomarkers
- From:Genomics & Informatics
2010;8(1):34-40
- CountryRepublic of Korea
- Language:English
-
Abstract:
Radiofrequency (RF) radiation might induce the transcription of a certain set of genes as other physical stresses like ionizing radiation and UV. To observe transcriptional changes upon RF radiation, we exposed WI-38, human lung fibroblast cell to 1763 MHz of mobile phone RF radiation at 60 W/kg of specific absorption rate (SAR) for 24h with or without heat control. There were no significant changes in cell numbers and morphology after exposure to RF radiation. Using quantitative RT-PCR, we checked the expression of three heat shock protein (HSP) (HSPA1A, HSPA6 and HSP105) and seven stress-related genes (TNFRSF11B, FGF2, TGFB2, ITGA2, BRIP1, EXO1, and MCM10) in RF only and RF/HS groups of RF-exposed cells. The expressions of three heat shock proteins and seven stress-related genes were selectively changed only in RF/HS groups. Based on the expression of ten genes, we could classify thermal and non-thermal effect of RF-exposure, which genes can be used as biomarkers for RF radiation exposure.
