Emergence of CTX-M-12 and A Novel CTX-M Type Extended-Spectrum beta-Lactamaseproducing Klebsiella pneumoniae.
10.3343/kjlm.2006.26.1.21
- Author:
Il Kwon BAE
1
;
Seok Hoon JEONG
;
Kyungwon LEE
;
Dongeun YONG
;
Jongwook LEE
;
Seong Geun HONG
;
Eui Chong KIM
;
Yeon Jun PARK
;
Jung Oak KANG
;
Young UH
;
Jong Hee SHIN
;
Wee Gyo LEE
;
Ji young AHN
;
Sung Hee LEE
;
Gun Jo WOO
;
Hyo Sun KWAK
Author Information
1. Department of Laboratory Medicine, Kosin University College of Medicine, Busan, Korea. kscpjsh@ns.kosinmed.or.kr
- Publication Type:Original Article
- Keywords:
Klebsiella pneumoniae;
Class A ESBL
- MeSH:
Agar;
Asia;
beta-Lactamases;
Cefotaxime;
Ceftazidime;
Diffusion;
Escherichia coli;
Klebsiella pneumoniae*;
Klebsiella*;
Korea;
Pneumonia;
Polymerase Chain Reaction;
Prevalence
- From:The Korean Journal of Laboratory Medicine
2006;26(1):21-26
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The aims of this study were to survey the nation-wide susceptibilities of Klebsiella pneumoniae isolates against ceftazidime and cefotaxime and to determine the prevalence of class A extended-spectrum beta-lactamases (ESBLs). METHODS: During the period of February to July 2004, K. pneumoniae isolates intermediate or resistant to ceftazidime and/or cefotaxime were collected from 12 hospitals in Korea. Antimicrobial susceptibilities were determined by the disk diffusion and the agar dilution methods and ESBL-production was by double-disk synergy test. Ceftazidime or cefotaxime-resistance determinants of the ESBLproducers were transfered to Escherichia coli J53 by transconjugation. Searches for class A ESBL genes were performed by PCR amplication. RESULTS: Among 212 clinical K. pneumoniae isolates, 172 (81%) isolates showed positive results in double-disk synergy test; the most prevalent ESBL was SHV-12 (n=104). Genes encoding ESBLs including SHV-2 (n=6), SHV-2a (n=17), CTX-M-3 (n=18), CTX-M-9 (n=6), CTX-M-12 (n=1), CTX-M- 14 (n=27), CTX-M-15 (n=3), and a novel CTX-M-type beta-lactamases were also detected. CONCLUSIONS: It is concluded that diversity of ESBLs in K. pneumoniae isolates are increasing in Korea. CTX-M-12 has never been reported in Asia, and a novel CTX-M-type ESBL has emerged.
