The expression of annexin I and thymosin beta4 in cervical cancer.
- Author:
Yu Sun LEE
1
;
Hye Won CHUNG
;
Hye Sung MOON
Author Information
1. Department of Obstetrics and Gynecology, School of Medicine,Ewha Womans University, Seoul, Korea. mhsmhs@ewha.ac.kr
- Publication Type:Original Article
- Keywords:
Annexin I;
Thymosin beta4;
Cervical cancer
- MeSH:
Annexin A1;
Biomarkers;
Blotting, Western;
Cervical Intraepithelial Neoplasia;
Cervix Uteri;
Female;
Humans;
Peptides;
RNA, Messenger;
Thymosin;
Uterine Cervical Neoplasms
- From:Korean Journal of Obstetrics and Gynecology
2008;51(3):313-323
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: The aim of this study was to compare the expression of annexin I and thymosin beta4 in invasive cervical cancer including normal cervix and CIN. METHODS: In Ewha Womans University Mokdong Hospital, normal cervical tissues were obtained from healthy women (n=10), from patients with cervical intraepithelial neoplasia (CIN, n=10) and from patients with cervical cancer (n=33). The expressions of annexin I and thymosin beta4 mRNA and protein were examined by quantitative competitive-PCR and by western blot analysis. The expressions of annexin I and thymosin beta4 protein were measured by western blot analysis with thymosin beta4 peptides non treated and treated SiHa cells. RESULTS: The expression of thymosin beta4 mRNA and protein in cervical cancer were higher than that in normal cervix (p<0.05). The expression of annexin I mRNA and protein were higher than that in normal cervix and CIN (p<0.05). Thymosin beta4 and annexin I mRNA expressions were not significantly correlated with cervical cancer stage, or size of the tumor (p>0.05). But thymosin beta4 and annexin I protein expressions were increased according to the cancer stage. The expression of annexin I was slightly higher in thymosin beta4 treated SiHa cells than that in nontreated SiHa cells. CONCLUSIONS: Our results suggest that overexpression of thymosin beta4 and annexin I may play roles in progression of invasive cervical cancer. Thymosin beta4 upregulates expression of annexin I in invasive cervical cancer. Therefore, thymosin beta4 and annexin I may be biological markers in detecting the progression of invasive cervical cancer, and their interaction is important in invasive cervical cancer.
