Genotyping of Giardia duodenalis Isolates from Dogs in Guangdong, China Based on Multi-Locus Sequence.
- Author:
Guochao ZHENG
1
;
Muhamd ALSARAKIBI
;
Yuanjia LIU
;
Wei HU
;
Qin LUO
;
Liping TAN
;
Guoqing LI
Author Information
1. College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China. gqli@scau.edu.cn
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
Giardia duodenalis;
dog;
multi-locus sequence typing;
Guangdong;
China
- MeSH:
Animals;
China;
Cluster Analysis;
Coinfection/parasitology/veterinary;
Cytoskeletal Proteins/genetics;
DNA, Protozoan/chemistry/genetics;
Dog Diseases/parasitology;
Dogs;
Genotype;
Giardia lamblia/*classification/*genetics/isolation & purification;
Giardiasis/parasitology/*veterinary;
Glutamate Dehydrogenase/genetics;
Molecular Sequence Data;
*Multilocus Sequence Typing;
Phylogeny;
Polymerase Chain Reaction;
RNA, Ribosomal, 18S/genetics;
Triose-Phosphate Isomerase/genetics
- From:The Korean Journal of Parasitology
2014;52(3):299-304
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study aimed to identify the assemblages (or subassemblages) of Giardia duodenalis by using normal or nested PCR based on 4 genetic loci: glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), beta-giardin (bg), and small subunit ribosomal DNA (18S rRNA) genes. For this work, a total of 216 dogs' fecal samples were collected in Guangdong, China. The phylogenetic trees were constructed with MEGA5.2 by using the neighbor-joining method. Results showed that 9.7% (21/216) samples were found to be positive; moreover, 10 samples were single infection (7 isolates assemblage A, 2 isolates assemblage C, and 1 isolate assemblage D) and 11 samples were mixed infections where assemblage A was predominant, which was potentially zoonotic. These findings showed that most of the dogs in Guangdong were infected or mixed-infected with assemblage A, and multi-locus sequence typing could be the best selection for the genotype analysis of dog-derived Giardia isolates.
