Protective Effects of Alpinia katsumadai Extract Against Oxidative Stress.
- Author:
Eul Jae LEE
1
;
Jeong Hee KIM
Author Information
1. Department of Oral Biochemistry and Institute of Oral Biology, College of Dentistry, Kyung Hee University, 130-701, Korea. jhkimh@khu.ac.kr
- Publication Type:Original Article
- Keywords:
medicinal plant extract;
protection;
reactive oxygen species
- MeSH:
1-Butanol;
Alpinia;
Cell Membrane;
Cell Proliferation;
Hexanes;
Hydrogen Peroxide;
Lipid Peroxidation;
Methanol;
Methylene Chloride;
Oxidative Stress;
Reactive Oxygen Species;
Water
- From:International Journal of Oral Biology
2011;36(3):149-154
- CountryRepublic of Korea
- Language:English
-
Abstract:
In the present study, total methanol extracts prepared from Alpinia katsumadai showed significant protective effects against the oxidative stress induced by hydrogen peroxide, UV-C or gamma-ray irradiation. These protective effects were substantially increased by treatment with 20~100 microg/ml of the extract. The A. katsumadai total methanol preparation was further fractionated into n-hexane, dichloromethane, ethylacetate, n-butanol and water fractions. Among these five fractions, the ethylacetate and butanol fractions of A. katsumadai showed the strongest protective effects against oxidative stress induced by UV-C and gamma-ray irradiation. These fractions also showed high DPPH radical scavenging and lipid peroxidation inhibitory activities. In addition, both fractions displayed cell proliferation activation effects, as evidenced by significant increases in colony formation. Our current data thus suggest that the mechanisms underlying the protective effects of A. katsumadai against oxidative damage may include radical scavenging, protection against cell membrane damage and stimulation of cell proliferation.
