Neuroprotective Effect of Growth Hormone in Neonatal Rat with Hypoxic Ischemic Brain Injury.
- Author:
Jae Hong YU
;
Kyu Sang SONG
;
Ren Zhe ANN
- Publication Type:Original Article
- Keywords:
Hypoxic ischemic brain injury;
Growth hormone;
Neuroprotection
- MeSH:
Animals;
Apoptosis;
Brain Injuries*;
Brain*;
Carotid Arteries;
Control Groups;
Ether;
Growth Hormone*;
Injections, Intraperitoneal;
Ligation;
Necrosis;
Neurons;
Neuroprotective Agents*;
Oxygen;
Rats*;
Rats, Sprague-Dawley
- From:Journal of Korean Society of Pediatric Endocrinology
2001;6(2):147-153
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the neuroprotective effect of growth hormone(GH) on neuronal cell necrosis and apoptosis at 1 week and 3 weeks after hypoxic ischemic brain injury. METHODS: Sprague-Dawley rats, seven-day-old, were used. Rats were anesthetized with ether less than 5 minutes. The right carotid artery was cut between double ligation. And then, rats were allowed to recover for 30 minutes followed by exposure to 8% oxygen at 37 degrees C for 2 hours for hypoxic ischemic insult. The study group was divided into 2 groups, control group(N=3) and GH treated group(N=3). GH treated group received intraperitoneal injection of GH 1 IU 2 hours after hypoxic ischemic insult following daily adminstration as same dose for 5 days. Rats were decapitated at 1 week and 3 weeks after hypoxic ischemic brain injury. After then, right hippocampal CA1 and CA3 neurons of rat brains were examined. RESULTS: Necrosis was significantly less in GH treated group than control group, and was more prominent at 3 weeks in both groups. The apoptosis was not found in GH treated and control group. CONCLUSION: GH has a neuroprotective effect on neuronal cell deaths(especially necrosis) from 1 week to 3 week after hypoxic ischemic insult in neonatal rat.
