Functional Polymorphism in the Promoter Region of Matrix Metalloproteinase-9 is Strongly Associated with Acute Myocardial Infarction.
10.4070/kcj.2005.35.2.192
- Author:
Pum Joon KIM
1
;
Kiyuk CHANG
;
Yoon Seok KOH
;
Ki Bae SEUNG
;
Sang Hong BAEK
;
Woo Seung SHIN
;
Sang Hyun LIM
;
Jae Hyung KIM
;
Soon Jo HONG
;
Kyu Bo CHOI
Author Information
1. Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea.
- Publication Type:Case Report
- Keywords:
Matrix metalloproteinase-9;
Polymorphism;
Acute myocardial infarction
- MeSH:
Acute Coronary Syndrome;
Coronary Artery Disease;
Enzyme-Linked Immunosorbent Assay;
Humans;
Incidence;
Logistic Models;
Matrix Metalloproteinase 9*;
Myocardial Infarction*;
Plaque, Atherosclerotic;
Polymerase Chain Reaction;
Polymorphism, Restriction Fragment Length;
Polymorphism, Single Nucleotide;
Promoter Regions, Genetic*;
Risk Factors;
Rupture
- From:Korean Circulation Journal
2005;35(2):192-196
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND AND OBJECTIVES: Matrix metalloproteinase-9 (MMP-9) plays an important role in the genesis of atherosclerotic plaque rupture and acute coronary syndrome (ACS), including an acute myocardial infarction (AMI). A single nucleotide polymorphism (SNP) in the MMP-9 promoter (-1562C>T) has recently been identified. This study investigated whether the SNP of the MMP-9 promoter is a significant risk factor for an AMI due to plaque rupture and if SNPs affect the transcription of the gene that elevates the MMP-9 expression level. SUBJECTS AND METHODS: A polymerase chain reaction, followed by a restriction fragment length polymorphism analysis, was performed in 173 control participants and 206 AMI patients. The serum levels of MMP-9 in the groups with or without the SNP were measured, using ELISA, and compared. RESULTS: There was a significantly higher incidence of the -1562C>T MMP-9 polymorphism in the AMI compared to the control group (27.6% vs. 17.9%, p=0.04). A multiple logistic regression analysis of the risk factors for coronary artery disease and the MMP-9 polymorphism showed the MMP-9 polymorphism to be an important factor in the prediction of an AMI (odds ratio 1.67, 95% confidence interval 1.02-2.67, p=0.04). CONCLUSION: The -1562C>T polymorphism in the MMP-9 promoter is a definite risk factor for an AMI, and is associated with elevated MMP-9 expression. These results suggest that a SNP in the MMP-9 promoter is strongly associated with an Acute Myocardial Infarction.
