Methylation of P16 and hMLH1 in Gastric Carcinoma.
10.5230/jkgca.2005.5.4.228
- Author:
Gi Young SUNG
1
;
Kyung Hwa CHUN
;
Jin Jo KIM
;
Hyung Min CHIN
;
Wook KIM
;
Cho Hyun PARK
;
Seung Man PARK
;
Keun Woo LIM
;
Woo Bae PARK
;
Seung Nam KIM
;
Hae Myung JEON
Author Information
1. Department of Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea. hmjeon@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Gastric carcinoma;
P16;
hMLH1;
Methylation;
Gene
- MeSH:
Adenocarcinoma;
Classification;
DNA;
Genes, p16;
Methylation*;
Polymerase Chain Reaction;
Stomach Neoplasms
- From:Journal of the Korean Gastric Cancer Association
2005;5(4):228-237
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: We investigated the impacts of the methylation states of the P16 and the hMLH1 genes on pathogenesis and genetic expression of stomach cancer and their relationships with Helicobater pylori infection, and with other clinico-pathologic factors. MATERIAL AND METHODS: In our study, to detect protein expression and methylation status of the P16 and the hMLH1 genes in 100 advanced gastric adenocarcinomas, used immunohistochemical staining and methylation-specific PCR (MSP) and direct automatic genetic sequencing analysis. RESULTS: Methylation of the P16 gene was observed in 19 out of 100 cases (19%) and in the 18 of those cases (94.7%) loss of protein expression was seen. We were sble to show that loss of P16 gene expression was related to methylation of the P16 gene (kappa coefficient=0.317, P=0.0011). Methylation of the hMLH1 gene was observed in 27 cases (27%), and in 24 cases of those 27 cases (88.8%), loss of protein expression was seen, which suggested that loss of protein expression in the hMLH1 gene is related to methylation of hMLH1 gene (kappa coefficient=0.675, P<0.0001). Also methylation of the hMLH1 gene was related to age, size of the mass, and Lauren's classification. CONCLUSION: We found that methylation of DNA plays an important role in inactivation of the P16 and the hMLH1 genes. The methylation of the hMLH1 genes is significantly related to age, size of the mass, and Lauren's classification.
