Mutation of the Chk1 Gene in Gastric Cancers with Microsatellite Instability.
10.5230/jkgca.2005.5.4.260
- Author:
Jong Heun LEE
1
;
Young Gu CHO
;
Jae Whie SONG
;
Cho Hyun PARK
;
Suk Woo NAM
;
Sug Hyung LEE
;
Nam Jin YOO
;
Jung Young LEE
;
Won Sang PARK
Author Information
1. Department of Pathology, The Catholic University of Korea College of Medicine, Seoul, Korea. wonsang@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Gastric cancer;
Microsatellite instability;
Mutation;
Cell cycle
- MeSH:
Cell Cycle;
Cell Cycle Checkpoints;
DNA Damage;
Frameshift Mutation;
Microdissection;
Microsatellite Instability*;
Microsatellite Repeats*;
Protein Kinases;
Stomach Neoplasms*
- From:Journal of the Korean Gastric Cancer Association
2005;5(4):260-265
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The protein kinase Chk1 is required for cell cycle arrest in response to DNA damage and is shown to play an important role in the G2/M checkpoint. The aim of this study was to investigate the relationship between microsatellite instability and frameshift mutation of the Chk1 gene in gastric cancers. MATERIALS AND METHODS: The microsatellite instability was analyzed in 95 primary gastric carcinomas by using microdissection and 6 microsatellite markers. We also performed single strand conformational polymorphism and sequencing to detect frameshift mutation of the Chk1 gene. RESULTS: We found positive microsatellite instability in 19 (20%) of the 95 gastric cancers, 13 high- and 6 low-frequency microsatellite instability cases. The frameshift mutation of Chk1, which resulted in a truncated Chk1 protein, was detected in two high-frequency microsatellite instability cases. CONCLUSION: These data suggest that the microsatellite instability may contribute to the development of gastric carcinomas through inactivation of Chk1.
