Histological Study of the Effect of Flos Carthami on Rabbit Atherosclerosis Following Cholesterol Diet.
10.11637/kjpa.1998.11.1.139
- Author:
Nak Jin PARK
1
;
Nak Won SOHN
;
Hak In LEE
Author Information
1. Department of Anatomy, College of Oriental Medicine, KyungSan University, Korea.
- Publication Type:Original Article
- Keywords:
Flos Carthami;
atherosclerosis;
cholesterol;
hypercholesterolemia
- MeSH:
Alcian Blue;
Animals;
Aorta;
Atherosclerosis*;
Body Weight;
Cholesterol*;
Collagen;
Diet*;
Hematoxylin;
Hydrogen-Ion Concentration;
Hypercholesterolemia;
Plaque, Atherosclerotic;
Rabbits;
Tunica Intima;
Tunica Media
- From:Korean Journal of Physical Anthropology
1998;11(1):139-145
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study was aimed to investigate the effect of Flos Carthami on the atherosclerosis in rabbit induced by cholesterol diet. 24 rabbits were divided by 3 groups; normal control group, CH group, and CH+FC group. The normal control group was fed with the normal pellet diet. The CH group was fed with pellet diet including 4% cholesterol, and the CH+FC group was fed with pellet diet including 4% cholesterol and 4% Flos Carthami dry -extract powder about 100g diet per 1 kg body weight per a day. After 12 weeks, animals were sacrificed and a piece of ascending aorta was collected. Tissue was sectioned 8 micrometer thickness and sections were stained with hematoxylin -eosin, alcian blue pH 2.5, aldehyde -fuchsin, and Van Gieson 's trichrome method. In CH+FC group, atheroma and mucoprotein formation on tunica intima of the ascending aorta was reduced, and lesion of elastic and collagen fibers in tunica media was also attenuated with respect to that in CH group. According to this result, it is considered that Flos Carthami has a preventing effect on atherosclerosis or a control effect on hypercholesterolemia. But distinct mechanism of action is still unclear.
