Immunohistological Study on the Distribution of Type I Collagen of the Skin in Adriamycin Treated Rats.
10.11637/kjpa.1998.11.1.155
- Author:
Ho Sam CHUNG
1
;
Kyun Ho CHANG
Author Information
1. Department of Anatomy, College of Medicine, Hanyang University and Institute of Biomedical Sciences, Korea.
- Publication Type:Original Article
- Keywords:
adriamycin;
skin;
type I collagen
- MeSH:
Animals;
Collagen Type I*;
Dermis;
Doxorubicin*;
Goats;
Immunoglobulin G;
Peroxidase;
Rats*;
Skin*
- From:Korean Journal of Physical Anthropology
1998;11(1):155-163
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The present study was designed to detect the distribution of the type I collagens in the three regions, papillary layer, reticular layer and subcutaneous layer of skin in the rats after administration of adriamycin. Intraperitoneally, 1 injections of 5 mg/kg of adriamycin for every 3 days were given and sacrificed 12 hours, 24 hours, 3 days and 1 week later respectively. For the immunoreactions of type I collagen, the specimens were incubated with rabbit anti rat type I collagen polyclonal antibody as primary antibody and biotinylated Goat anti rabbit IgG antibody as secondary antibody. And ABC peroxidase procedure was used. The reactions of type I collagen in the dermis of adriamycin treated rat skin were decreased from 12 hours to 3 day and from 7 day the immunoreactions are constant as like that of normal rats. Adriamycin seems to be suppressing normal components of type I collagen in rat skin.
