A Rapid Diagnostic Test for Toxoplasmosis using Recombinant Antigenic N-terminal Half of SAG1 Linked with Intrinsically Unstructured Domain of GRA2 Protein.
- Author:
Kyoung Ju SONG
1
;
Zhaoshou YANG
;
Chom Kyu CHONG
;
Jin Soo KIM
;
Kyung Chan LEE
;
Tong Soo KIM
;
Ho Woo NAM
Author Information
1. Department of Parasitology, Catholic Institute of Parasitic Disease, College of Medicine, Catholic University of Korea, Seoul 137-701, Korea. howoo@catholic.ac.kr
- Publication Type:Original Article ; Evaluation Studies ; Research Support, Non-U.S. Gov't
- Keywords:
Toxoplasma gondii;
serodiagnosis;
rapid diagnostic test;
recombinant GST-GRA2-SAG1A;
ELISA;
intrinsically unstructured domain
- MeSH:
Adolescent;
Adult;
Amino Acid Sequence;
Antibodies, Protozoan/*blood;
Antigens, Protozoan/genetics/*immunology;
Child;
Child, Preschool;
Female;
Humans;
Infant;
Male;
Molecular Sequence Data;
Protozoan Proteins/genetics/*immunology;
Recombinant Fusion Proteins;
Reproducibility of Results;
Republic of Korea/epidemiology;
Sensitivity and Specificity;
Serologic Tests;
Time Factors;
Toxoplasma/genetics/*immunology/isolation & purification;
Toxoplasmosis/*diagnosis/epidemiology/parasitology;
Uganda/epidemiology;
Young Adult
- From:The Korean Journal of Parasitology
2013;51(5):503-510
- CountryRepublic of Korea
- Language:English
-
Abstract:
Toxoplasma gondii is an apicomplexan parasite with a broad host range of most warm-blooded mammals including humans, of which one-thirds of the human population has been infected worldwide which can cause congenital defects, abortion, and neonatal complications. Here, we developed a rapid diagnostic test (RDT) for T. gondii infection. Antigenic N-terminal half of the major surface antigen (SAG1) was linked with intrinsically unstructured domain (IUD) of dense granule protein 2 (GRA2). The recombinant GST-GRA2-SAG1A protein was successfully expressed and purified as 51 kDa of molecular weight. Furthermore, antigenicity and solubility of the rGST-GRA2-SAG1A protein were significantly increased. The overall specificity and sensitivity of GST-GRA2-SAG1A loaded RDT (TgRDT) were estimated as 100% and 97.1% by comparing with ELISA result which uses T. gondii whole cell lysates as the antigen. The TgRDT tested with Uganda people sera for field trial and showed 31.9% of seroprevalence against T. gondii antibody. The TgRDT is proved to be a kit for rapid and easy to use with high accuracy, which would be a suitable serodiagnostic tool for toxoplasmosis.
