Phylogenetic Analysis of Ruminant Theileria spp. from China Based on 28S Ribosomal RNA Gene.
- Author:
Huitian GOU
1
;
Guiquan GUAN
;
Miling MA
;
Aihong LIU
;
Zhijie LIU
;
Zongke XU
;
Qiaoyun REN
;
Youquan LI
;
Jifei YANG
;
Ze CHEN
;
Hong YIN
;
Jianxun LUO
Author Information
1. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Key Laboratory of Grazing Animal Diseases MOA, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu 730046, P. R. China. vectorparasit@126.com
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Theileria sp.;
28S rRNA;
phylogeny;
cattle;
sheep;
China
- MeSH:
Animals;
Base Sequence;
China;
DNA, Ribosomal/chemistry/genetics;
Molecular Sequence Data;
Phylogeny;
RNA, Ribosomal, 28S/genetics;
Ruminants;
Sequence Alignment;
Sequence Analysis, DNA/veterinary;
Theileria/*classification/genetics/isolation & purification;
Theileriasis/*parasitology
- From:The Korean Journal of Parasitology
2013;51(5):511-517
- CountryRepublic of Korea
- Language:English
-
Abstract:
Species identification using DNA sequences is the basis for DNA taxonomy. In this study, we sequenced the ribosomal large-subunit RNA gene sequences (3,037-3,061 bp) in length of 13 Chinese Theileria stocks that were infective to cattle and sheep. The complete 28S rRNA gene is relatively difficult to amplify and its conserved region is not important for phylogenetic study. Therefore, we selected the D2-D3 region from the complete 28S rRNA sequences for phylogenetic analysis. Our analyses of 28S rRNA gene sequences showed that the 28S rRNA was useful as a phylogenetic marker for analyzing the relationships among Theileria spp. in ruminants. In addition, the D2-D3 region was a short segment that could be used instead of the whole 28S rRNA sequence during the phylogenetic analysis of Theileria, and it may be an ideal DNA barcode.
